Complete Genome Analysis of Highly Pathogenic Non-O1/O139 Vibrio cholerae Isolated From Macrobrachium rosenbergii Reveals Pathogenicity and Antibiotic Resistance-Related Genes.
Complete Genome Analysis of Highly Pathogenic Non-O1/O139 Vibrio cholerae Isolated From Macrobrachium rosenbergii Reveals Pathogenicity and Antibiotic Resistance-Related Genes.
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DOI：
10.3389/fvets.2022.882885
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发表时间：
2022
影响因子：
3.2
通讯作者：
中科院分区：
文献类型：
--
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非O 1/O 139霍乱弧菌是一种高致病力的病原体，可导致各种水生动物的大量死亡。为了研究罗氏沼虾非O 1/O 139霍乱弧菌GXFL 1 -4的致病性和耐药性，对罗氏沼虾非O 1/O 139霍乱弧菌GXFL 1 -4进行全基因组测序。结果表明，该菌的基因组包含2条环状染色体和1个质粒，质粒大小为4，282，243 bp，含有3，869个编码基因。其中，3，047、2，659和3，661个基因分别被注释在Orthopathic Genes（COG）、Gene Ontology（GO）和京都基因和基因组百科全书（KEGG）中。此外，基于毒力因子数据库（VFDB）数据库，预测了372个潜在的毒力基因，如II、III、IV和VI型分泌系统相关基因，鞭毛基因，菌毛形成或运动相关基因。在综合抗生素耐药数据库（CARD）数据库中的Blast结果显示，该菌株含有外排泵复合体抗生素耐药基因、抗生素耐药基因簇基因等27大类148个抗生素耐药相关基因。病原体-宿主相互作用（PHI）数据库注释了320个与病原体-宿主相互作用相关的基因，如T3 SS、毒力调节因子、转录调节因子、双组分反应调节因子等。全基因组分析表明，非O 1/O 139霍乱弧菌GXFL 1 -4株可能具有复杂的致病和耐药分子机制。本研究提供了与霍乱弧菌致病性和耐药性相关的非O 1/O 139基因的丰富信息，将有助于了解霍乱弧菌的致病机制以及制定防治策略。
Non-O1/O139 Vibrio cholerae is a highly virulent pathogen that causes mass mortalities of various aquatic animals. In the present study, we sequenced the whole genome of non-O1/O139 V. cholerae GXFL1-4, isolated from Macrobrachium rosenbergii, to reveal the pathogenicity and antibiotic resistance. The result showed its genome contained two circular chromosomes and one plasmid with a total size of 4,282,243 bp, which harbored 3,869 coding genes. Among them, 3,047, 2,659, and 3,661 genes were annotated in the Clusters of Orthologous Genes (COG), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG), respectively. In addition, 372 potential virulence genes were predicted based on the Virulence Factor Database (VFDB) database, such as type II, III, IV, and VI secretion systems related genes, flagella genes, and pilus formation or motility-related genes. Blast results in the Comprehensive Antibiotic Resistance Database (CARD) database showed that the strain contained 148 antibiotic resistance-related genes belonging to 27 categories, such as efflux pump complex antibiotic resistance genes and antibiotic resistance gene cluster genes. The Pathogen-Host Interaction (PHI) database annotated 320 genes related to pathogen-host interaction, such as T3SS, virulence regulatory factors, transcriptional regulators, and two-component response regulator related genes. The whole-genome analysis suggested that the pathogenic non-O1/O139 V. cholerae strain GXFL1-4 might have a complex molecular mechanism of pathogenicity and antibiotic resistance. This study provides a wealth of information about non-O1/O139 V. cholerae genes related to its pathogenicity and drug resistance and will facilitate the understanding of its pathogenesis as well as the development of prevention and treatment strategies for the pathogen.
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影响因子：
4.1
作者：
Shang, Xuan;Cheng, Hanhua;Zhou, Rongjia
通讯作者：
Zhou, Rongjia
影响因子：
9.4
作者：
Namdari, H;Klaips, CR;Hughes, JL
通讯作者：
Hughes, JL
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
14.9
作者：
Besemer, J;Lomsadze, A;Borodovsky, M
通讯作者：
Borodovsky, M
影响因子：
4
作者：
Lim, H. J.;Lee, E. -H.;Son, A.
通讯作者：
Son, A.