Subtractive cloning of cDNA from Aspergillus oryzae differentially regulated between solid-state culture and liquid (submerged) culture
Subtractive cloning of cDNA from Aspergillus oryzae differentially regulated between solid-state culture and liquid (submerged) culture
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DOI：
10.1007/s00294-002-0314-y
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发表时间：
2002-07-01
期刊：
影响因子：
2.5
通讯作者：
Akita, O
中科院分区：
文献类型：
--
作者：
Akao, T;Gomi, K;Akita, O
在固态培养（SC）中，曲霉菌表现出液体（浸没）培养（LC）中不存在的特性，如高水平的酶的产生和分泌以及菌丝分化和无性发育。据预测，许多基因参与的特性，A。在SC和LC之间差异表达。我们用双向cDNA消减杂交技术构建了两个消减cDNA文库，以分离和鉴定这些基因。在这些基因中，我们鉴定了SC中上调或特异的基因，如AOS（A。这些基因在SC中表达下调或不表达，如AOL（A. HPLC特异性）系列。测序分析表明，AOS系列和AOL系列含有编码胞外和胞内酶和转运蛋白的基因。然而，有一半是功能上未分类的核苷酸序列。此外，通过表达谱，AOS系列包括两组。这些基因产物在SC中的分子功能和生理作用有待进一步研究。
In solid-state cultures (SC), Aspergillus oryzae shows characteristics such as high-level production and secretion of enzymes and hyphal differentiation with asexual development which are absent in liquid (submerged) culture (LC). It was predicted that many of the genes involved in the characteristics of A. oryzae in SC are differentially expressed between SC and LC. We generated two subtracted cDNA libraries with bi-directional cDNA subtractive hybridizations to isolate and identify such genes. Among them, we identified genes upregulated in or specific to SC, such as the AOS (A. oryzae SC-specific gene) series, and those downregulated or not expressed in SC, such as the AOL (A. oryzae LC-specific) series. Sequencing analyses revealed that the AOS series and the AOL series contain genes encoding extra- and intracellular enzymes and transport proteins. However, half were functionally unclassified by nucleotide sequences. Also, by expression profile, the AOS series comprised two groups. These gene products' molecular functions and physiological roles in SC await further investigation.