Saline stress alters the temporal patterns of xylem differentiation and alternative oxidase expression in developing soybean roots
Saline stress alters the temporal patterns of xylem differentiation and alternative oxidase expression in developing soybean roots
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DOI：
10.1104/pp.117.2.695
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发表时间：
1998-06-01
期刊：
影响因子：
7.4
通讯作者：
Massa, EM
中科院分区：
文献类型：
--
作者：
Hilal, M;Zenoff, AM;Massa, EM
采用生物化学和形态计量学相结合的方法，研究了盐胁迫对发育中大豆(Glyine max L.)分化带的影响。树根。在生长在对照或添加盐的基质中的幼苗，在d3至d14期间，我们研究了(A)呼吸交替氧化酶(AOX)能力的时间演变与组织印迹免疫印迹分析AOX蛋白的表达和定位的相关性；(B)参与木质化的过氧化物酶活性的时间演变和组织定位；以及(C)通过光学显微镜观察和图像数字化量化的结构变化。结果表明，盐胁迫延缓了初生木质部的分化。AOX表达的时间模式有相应的延迟，这与AOX蛋白的木质部特异性定位以及该酶与木质部发育有关的想法是一致的。此外，盐胁迫对次生木质部的发育也有促进作用。然而，初级和次生木质部中过氧化物酶活性的时间模式并没有受到盐处理的影响。因此，在AOX表达和木质部发育的时间模式中，盐胁迫对根的影响是特异的。
We conducted a coordinated biochemical and morphometric analysis of the effect of saline conditions on the differentiation zone of developing soybean (Glycine max L.) roots. Between d 3 and d 14 for seedlings grown in control or NaCl-supplemented medium, we studied (a) the temporal evolution of the respiratory alternative oxidase (AOX) capacity in correlation with the expression and localization of AOX protein analyzed by tissue-print immunoblotting; (b) the temporal evolution and tissue localization of a peroxidase activity involved in lignification; and (c) the structural changes, visualized by light microscopy and quantified by image digitization. The results revealed that saline stress retards primary xylem differentiation. There is a corresponding delay in the temporal pattern of AOX expression, which is consistent with the xylem-specific localization of AOX protein and the idea that this enzyme is linked to xylem development. An NaCl-induced acceleration of the development of secondary xylem was also observed. However, the temporal pattern of a peroxidase activity localized in the primary and secondary xylem was unaltered by NaCl treatment. Thus, the NaCl-stressed root was specifically affected in the temporal patterns of AOX expression and xylem development.