Human Cytoplasmic Actin Proteins Are Encoded by a Multigene Family
Human Cytoplasmic Actin Proteins Are Encoded by a Multigene Family
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人类细胞质肌动蛋白由多基因家族编码
DOI：
10.1128/mcb.2.6.674-684.1982
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发表时间：
1982
影响因子：
5.3
通讯作者：
L. Kedes
中科院分区：
文献类型：
--
作者：
J. Engel;P. Gunning;L. Kedes
我们鉴定了从克隆人类 DNA 文库中分离出的 9 个人类肌动蛋白基因（Engel 等人，Proc. Natl. Acad. Sci. U.S.A. 78：4674-4678，1981）。对每个克隆的肌动蛋白基因与 α-、β-和 γ-肌动蛋白 mRNA 之间形成的杂交体的热稳定性测量表明，只有一个克隆与肌节肌动蛋白 mRNA 最同源，而其余 8 个克隆与细胞质肌动蛋白 mRNA 最同源。通过以下标准，我们表明这九个克隆代表九个不同的肌动蛋白基因位点，而不是不同的等位基因或单个基因的不同部分：（i）编码区的限制性酶图谱不同； (ii)每个克隆含有足够的编码区来编码整个肌动蛋白基因的全部或大部分； (iii)每个克隆含有与克隆的鸡β-肌动蛋白cDNA编码区的5'和3'端同源的序列。因此，我们得出结论，人类细胞质肌动蛋白是由多基因家族编码的。
We characterized nine human actin genes that we isolated (Engel et al., Proc. Natl. Acad. Sci. U.S.A. 78:4674-4678, 1981) from a library of cloned human DNA. Measurements of the thermal stability of hybrids formed between each cloned actin gene and α-, β-, and γ-actin mRNA demonstrated that only one of the clones is most homologous to sarcomeric actin mRNA, whereas the remaining eight clones are most homologous to cytoplasmic actin mRNA. By the following criteria we show that these nine clones represent nine different actin gene loci rather than different alleles or different parts of a single gene: (i) the restriction enzyme maps of the coding regions are dissimilar; (ii) each clone contains sufficient coding region to encode all or most of an entire actin gene; and (iii) each clone contains sequences homologous to both the 5′ and 3′ ends of the coding region of a cloned chicken β-actin cDNA. We conclude, therefore, that the human cytoplasmic actin proteins are encoded by a multigene family.