Experimental Investigation on the Formation of Dense-Branching-Morphology-Like Colonies in Bacteria
Experimental Investigation on the Formation of Dense-Branching-Morphology-Like Colonies in Bacteria
复制标题
细菌密集分支形态样菌落形成的实验研究
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
M. Matsushita
中科院分区：
文献类型：
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作者：
J. Wakita;Ismael Rafols;Hiroto Itoh;T. Matsuyama;M. Matsushita
我们通过实验研究了细菌菌落的形成模式。我们特别检查了枯草芽孢杆菌的 DBM（密集分支形态学）样菌落。通过显微镜观察发现，每个生长枝条的顶端都聚集着许多活跃的细菌细胞并形成群体。树枝彼此排斥，有时在向外生长时会分裂。宏观上证实，当养分浓度C n 增加时，平均分枝宽度和平均分枝间隙均系统地减小，但它们的比值保持不变，近似为1。还发现分支长度的分布非常接近指数，表明分支的尖端分裂是随机发生的。
We have investigated experimentally the pattern formation of bacterial colonies. We have especially examined DBM (dense-branching-morphology)-like colonies of bacterial species Bacillus subtilis . It was found from microscopic observations that many active bacterial cells collect and make a group on the tip of each growing branch. Branches repel each other and split sometimes as they grow outward. It was confirmed macroscopically that both averaged branch width and averaged branch gap decrease systematically when increasing the nutrient concentration C n , while their ratio remains unchanged with the approximate value of one. It was also found that the distribution of branch lengths is very close to exponential, suggesting that the tip-splitting of branches takes place at random.