A new electrochemical aptasensor based on electrocatalytic property of graphene toward ascorbic acid oxidation.
A new electrochemical aptasensor based on electrocatalytic property of graphene toward ascorbic acid oxidation.
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DOI：
10.1016/j.talanta.2014.12.011
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发表时间：
2015-03
期刊：
影响因子：
6.1
通讯作者：
Liang Wu;Erhu Xiong;Yue Yao;Xia Zhang;Xiaohua Zhang;Jinhua Chen
中科院分区：
文献类型：
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作者：
Liang Wu;Erhu Xiong;Yue Yao;Xia Zhang;Xiaohua Zhang;Jinhua Chen
基于石墨烯（GN）对抗坏血酸（AA）氧化的上级电催化性能，开发了一种新型的电化学适体传感器。在这里，三磷酸腺苷（ATP）被用作模型，以证明开发的适体传感器的性能。简言之，GN通过π-π堆积相互作用连接到巯基化ATP结合适体（阿坝）修饰的金电极，导致AA的显著氧化信号。在ATP存在下，ATP-ABA复合物的形成导致GN从传感界面释放，导致AA的氧化峰电流急剧下降，相关峰电位明显正移。以AA氧化峰电流和峰电位的变化值作为响应信号，可灵敏地检测ATP。这是第一次证明GN作为纳米催化剂在放大的适体传感器中的应用。GN作为纳米催化剂有望成为DNA电化学生物传感器中非常有前途的放大元件。
Based on the superior electrocatalytic property of graphene (GN) toward ascorbic acid (AA) oxidation, a new electrochemical aptasensor has been developed. Here, adenosine triphosphate (ATP) is used as the model to demonstrate the performance of the developed aptasensor. Briefly, GN is attached to the thiolated ATP binding aptamer (ABA) modified gold electrode through π-π stacking interaction, resulting in a significant oxidation signal of AA. In the presence of ATP, the formation of ATP-ABA complex leads to the release of GN from sensing interface, resulting in a sharp decrease of the oxidation peak current of AA and an obviously positive shift of the related peak potential. Taking both the change values of the peak current and peak potential of AA oxidation as the response signals, ATP can be detected sensitively. This is the first time to demonstrate the application of GN as the nanocatalyst in an amplified aptasensor. It can be expected that GN, as nanocatalyst, should become the very promising amplifying-elements in DNA-based electrochemical biosensors.