Modulation of JE/MCP-1 expression in dermal wound repair.
Modulation of JE/MCP-1 expression in dermal wound repair.
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发表时间：
1995
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通讯作者：
L. DiPietro;P. Polverini;S. Rahbe;E. Kovacs
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作者：
L. DiPietro;P. Polverini;S. Rahbe;E. Kovacs
组织巨噬细胞在伤口修复中起着重要作用，但影响巨噬细胞迁移到伤口床的参数还没有很好地理解。为了更好地理解巨噬细胞募集的过程，在皮肤伤口修复的鼠模型中检查了单核细胞趋化蛋白1（JE/MCP-1）的鼠同源物JE的产生。在创伤后12小时和1天（24小时），在真皮穿孔伤口中发现高水平的JE/MCP-1 mRNA; mRNA水平缓慢下降至第21天检测不到。伤口原位杂交分析显示，JE/MCP-1主要表达的单核细胞和巨噬细胞样细胞，以及偶尔成纤维细胞和其他间质细胞。为了将JE/MCP-1产生与巨噬细胞迁移相关联，对巨噬细胞浸润到伤口床中进行定量。伤口内的巨噬细胞数量在第3天增加到最大值（每个高倍视野11.3 +/-4.5个巨噬细胞），在第5天开始减少（每个高倍视野4.8 +/-1.9个巨噬细胞），并在第10天达到接近基线（每个高倍视野3.0 +/-1.1个巨噬细胞）。结果表明，伤口内JE/MCP-1的产生与巨噬细胞浸润的时间进程和分布密切相关，最大JE/MCP-1 mRNA水平出现在最大巨噬细胞浸润前1至2天。结果支持JE/MCP-1在伤口巨噬细胞募集中的作用，并表明巨噬细胞通过产生JE/MCP-1，可以维持将额外的单核细胞和巨噬细胞募集到损伤部位。
The tissue macrophage plays a prominent role in wound repair, yet the parameters that influence macrophage migration into the wound bed are not well understood. To better understand the process of macrophage recruitment, the production of JE, the murine homologue of monocyte chemoattractant protein 1(JE/MCP-1), was examined in a murine model of dermal wound repair. High levels of JE/MCP-1 mRNA were found in dermal punch wounds at 12 hours and 1 day (24 hours) after wounding; mRNA levels slowly decreased to undetectable by day 21. In situ hybridization analysis of wounds revealed that JE/MCP-1 was predominantly expressed by monocytic and macrophage-like cells, as well as by occasional fibroblasts and other interstitial cells. To correlate JE/MCP-1 production with macrophage migration, macrophage infiltration into the wound bed was quantitated. The number of macrophages within the wound increased to a maximum at day 3 (11.3 +/- 4.5 macrophages per high power field), began to decrease at day 5 (4.8 +/- 1.9 macrophages per high power field), and reached near base line at day 10 (3.0 +/- 1.1 macrophages per high power field). The results demonstrate that JE/MCP-1 production within wounds is closely linked to the time course and distribution of macrophage infiltration, with maximal JE/MCP-1 mRNA levels occurring 1 to 2 days before maximal macrophage infiltration. The results support a role for JE/MCP-1 in the recruitment of wound macrophages and suggest that macrophages, through the production of JE/MCP-1, may sustain the recruitment of additional monocytes and macrophages into sites of injury.