Temperature transitions of protein properties in human red blood cells
Temperature transitions of protein properties in human red blood cells
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DOI：
10.1016/s0006-3495(98)77759-8
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发表时间：
1998-12-01
影响因子：
3.4
通讯作者：
Chien, S
中科院分区：
文献类型：
--
作者：
Artmann, GM;Kelemen, C;Chien, S
人红细胞（RBC）在36.4 ℃的转变温度（T-c）下经历从阻塞到通过1.3 ± 0.2 μ m微量移液管的突然变化。对于重新密封的RBC血影，这种转变发生在28.3 ℃（T-g）。这些发现归因于血红蛋白从凝胶样到流体的弹性转变以及膜蛋白如血影蛋白的弹性转变。血影蛋白在T-g以上沿沿着抽吸的RBC舌显示均匀分布，而在T-g以下呈线性梯度。
Human red blood cells (RBC) undergo a sudden change from blocking to passing through 1.3 +/- 0.2-mu m micropipettes at a transition temperature (T-c) of 36.4 degrees C, For resealed RBC ghosts this transition occurs at 28.3 degrees C (T-g). These findings are attributed to an elastomeric transition of hemoglobin from being gel-like to a fluid and to an elastomeric transition of membrane proteins such as spectrin, Spectrin shows a uniform distribution along the aspirated RBC tongue above T-g in contrast to the linear gradient below T-g.