Alkaline hydrogen peroxide pretreatment, enzymatic hydrolysis and fermentation of sugarcane bagasse to ethanol
Alkaline hydrogen peroxide pretreatment, enzymatic hydrolysis and fermentation of sugarcane bagasse to ethanol
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DOI：
10.1016/j.fuel.2014.07.033
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发表时间：
2014-11-15
期刊：
影响因子：
7.4
通讯作者：
Costa, Aline C.
中科院分区：
文献类型：
--
作者：
Rabelo, Sarita C.;Andrade, Rafael R.;Costa, Aline C.
研究了碱性过氧化氢对甘蔗渣酶解和酿酒酵母发酵生产第二代乙醇的影响。因子设计用于确定颗粒尺寸减小的需要以及优化水解中的预处理条件和酶加载。确定了在预处理和水解阶段增加固体负荷的影响;进行了纯水解物的分批发酵，以及用甘蔗糖蜜浓缩的水解物的连续发酵。利用物料衡算确定了在不同操作条件下，以原料甘蔗渣的质量为单位得到的乙醇质量。预处理提高了甘蔗渣的酶消化率，而不需要事先减小尺寸。在最佳预处理条件（25 ℃，1.84 mL H2 O2/g甘蔗渣）和酶解条件（3.5 FPU/g甘蔗渣和25 CBU/g甘蔗渣）下，可得到416.7 kg葡萄糖/t原料甘蔗渣。纯水解液发酵，乙醇产率为187.85公斤/吨原料甘蔗渣。(C)2014爱思唯尔有限公司版权所有。
The pretreatment of sugarcane bagasse with alkaline hydrogen peroxide was evaluated for second generation ethanol production via enzymatic hydrolysis and fermentation using Saccharomyces cerevisiae. Factorial designs were used to determine the need for particle size reduction as well as to optimize pretreatment conditions and enzymes loadings in the hydrolysis. The influence of increasing solids loadings in the pretreatment and hydrolysis stages was determined; batch fermentation of pure hydrolysate, as well as continuous fermentation of hydrolysate concentrated with sugarcane molasses were performed. Furthermore, mass balances were used to determine the mass of ethanol obtained by mass of raw bagasse in different operational conditions. The pretreatment increased bagasse enzymatic digestibility without the need for prior size reduction. In the optimal pretreatment (1 h, 25 degrees C, 1.84 mL hydrogen peroxide/g bagasse) and hydrolysis conditions (3.5 FPU/g bagasse of cellulase and 25 CBU/g bagasse of beta-glucosidase), 416.7 kg glucose/ton of raw bagasse were obtained. Fermentation of pure hydrolysate led to an ethanol yield of 187.85 kg/ton of raw bagasse. (C) 2014 Elsevier Ltd. All rights reserved.