Laboratory Growth Systems in Biofilm Research
Laboratory Growth Systems in Biofilm Research
复制标题
生物膜研究中的实验室生长系统
DOI：
--
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
J. Wimpenny
中科院分区：
文献类型：
--
作者：
J. Wimpenny
我们统称为“生物膜”的生态系统种类繁多，反映在实验室中可用于培养它们的众多不同系统上。定义并讨论了原位系统、微观世界和实验室模型之间的关系。前两种方法代表了旨在评估特定生物膜的结构和功能的整体方法；最后一种是分析和还原主义，旨在分离生物膜的特定功能，以便了解可适用于一般生物膜的特性。一个模型的属性可以被完全理解，而这在自然生态系统中是不可能的，因为不可培养的物种可能在其结构和功能中发挥着未被认识但重要的作用。对一系列系统进行了审查。其中包括以不同方式暴露于营养物的简单表面，流动系统（如Robbins设备）和恒定剪切设备（如Rototorque和Fowler细胞粘附测量模块）。恒深膜发酵罐包括膜生物膜反应器和灌注式生物膜反应器。描述了一些微观世界的例子。从CDFF和流化床反应器的角度介绍了“稳态”生物膜的概念和价值。附录中列出了一些市售的薄膜发酵剂。
The huge variety of ecosystems that we collectively refer to as "biofilm" is reflected by the numerous different systems available to grow them in the laboratory. The relationship between in situ systems, microcosms and laboratory models is defined and discussed. The first two represent holistic approaches designed to assess the structure and function of particular biofilms: the last is analytical and reductionist, aiming to isolate specific functions of biofilms in order to understand properties that can apply to biofilm in general. Properties of a model can be completely understood whilst this is unlikely with natural ecosystems because of the possibility of unculturable species which could play an unrecognised but important part in its structure and function. A range of systems is reviewed. These include simple surfaces exposed to nutrient in different ways, flow systems such as the Robbins device and constant shear devices such as the Rototorque and the Fowler cell adhesion measurement module. The constant depth film fermenter (CDFF) is described as are membrane based models including the membrane biofilm and the perfused biofilm reactors. Some examples of microcosms are described. The concept and value of "steady state" biofilm is introduced in terms of the CDFF and of fluidised bed reactors. A number of commercially available film fermenters are listed in the appendix.