Metabolic Profiling and Post-harvest Behavior of "Dottato" Fig (Ficus carica L.) Fruit Covered With an Edible Coating From O. ficus-indica
Metabolic Profiling and Post-harvest Behavior of "Dottato" Fig (Ficus carica L.) Fruit Covered With an Edible Coating From O. ficus-indica
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DOI：
10.3389/fpls.2018.01321
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发表时间：
2018-09-05
影响因子：
5.6
通讯作者：
Martinelli, Federico
中科院分区：
文献类型：
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作者：
Allegra, Alessio;Gallotta, Alessandra;Martinelli, Federico
无花果果实在采后的生活中通常对一些生理病理学疾病高度敏感，例如软化和皮肤开裂。事实上，可食用涂层（EC）的使用已经在几种水果作物中进行了评估，以减少水果收获后的蒸腾作用，并保持水果的视觉质量。本研究的目的是确定采后的代谢反应处理从O puntia ficus-indica叶状枝的粘液提取物，使用非靶向代谢组学的方法。将涂覆的和未涂覆的（对照）水果密封在塑料袋中，并在4 ° C下储存7天。通过分析果实初级代谢和可溶性固形物（TSS）、可滴定酸（TA）、鲜重损失和硬度等品质指标，评价了EC对冷藏期间果实品质和品质性状的影响。结果表明，EC对保持果实鲜重、硬度有明显效果。逐步判别分析能够判别果实状况。丙氨酸、木酮糖、天冬氨酸、谷氨酸和2，5-二羟基吡嗪对可食涂膜果实与未涂膜果实的鉴别有显著作用。主成分分析（PCA）能够突出未处理和处理的水果之间的整体代谢变化的明显差异。EC的应用显著缓解了冷藏过程中大部分氨基酸含量的下降。与未处理的对照相比，EC处理引起了几种有机酸的变化，增加了碳水化合物和其他关键代谢物的量，如β-谷甾醇、甘油和尿嘧啶。这些结果清楚地表明EC对无花果采后代谢的显著影响，并揭示了这种处理的有益机制。
Fig fruits are usually highly sensitive to some physiopathological disorders during post-harvest life, such as softening and skin cracking. Indeed, the use of edible coating (EC) has been evaluated in several fruit crops to reduce fruit post-harvest transpiration and to maintain fruit visual quality. The aim of this study was to determine the post-harvest metabolic response of breba figs treated with mucilage extract from O puntia ficus-indica cladodes, using an untargeted metabolomic approach. Coated and non-coated (control) fruit were sealed in plastic bags, and stored at 4 degrees C for 7 days. The effect of the ECs on their quality fruit during cold storage and qualitative attributes were evaluated by analyzing the fruit primary metabolism and other qualitative parameters such as total soluble solids (TSS) content, titratable acidity (TA), fresh weight loss and firmness. Results underlined that EC was effective in maintaining fruit fresh weight, and fruit firmness. Stepwise discriminant analysis was able to discriminate fruit conditions. Alanine, xylulose, aspartic acid, glutamic, acid and 2,5-dihydroxypyrazine showed a significant role on discriminating edible coated fruit from untreated ones. Principal component analysis (PCA) was able to highlight clear differences in the overall metabolism changes between untreated and treated fruit. The application of EC significantly mitigated the decrease of most of the aminoacid content during cold storage. EC treatment caused the changes of several organic acids in comparison to untreated control, increasing the amount of carbohydrates and other key metabolites, such as beta-sitosterol, glycerol, and uracil. These results clearly showed the drastic effects of EC on fig metabolism during post-harvest and shed light on the beneficial mechanisms of this treatment.