IGF-II promotes mesoderm formation
IGF-II promotes mesoderm formation
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DOI：
10.1006/dbio.2000.9875
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发表时间：
2000-11-01
影响因子：
2.7
通讯作者：
Larue, L
中科院分区：
文献类型：
--
作者：
Morali, OG;Jouneau, A;Larue, L
IGF-II 在原肠胚小鼠胚胎的新生中胚层中含量丰富。其在这个发育阶段的功能尚不清楚。我们通过跟踪几种雄激素、双亲、孤雌和雄激素遗传 Igf2 -/- 鼠 ES 细胞系的体外和体内分化来研究它；这些细胞的内源性 IGF-II 水平有所不同，因为 Igf2 在小鼠胚胎的大多数组织中都是父系表达的。中胚层标志物的表达和随后的肌肉结构的形成与畸胎瘤形成和体外分化过程中的内源性IGF-II水平相关。此外，雄激素性Igf2 -/- ES细胞中Igf2的缺失导致中胚层发育严重受损，这表明在众多已知的印记基因中，雄激素性ES细胞的优先中胚层发育依赖于Igf2活性。在体外分化培养基中添加外源IGF-II导致中胚层标志物表达的特异性增加。因此，我们提出了一种新模型，其中 IGF-II 与其位于中胚层前体细胞表面的主要信号受体 IGF1R 的结合增加了中胚层细胞的形成。 (C) 2000 年学术出版社。
IGF-II is abundant in the nascent mesoderm of the gastrulating mouse embryo. Its function at this developmental stage is unknown. We investigated it by following the in vitro and in vivo differentiation of several androgenetic, biparental, parthenogenetic, and androgenetic Igf2 -/- murine ES cell lines; these cells differed in endogenous IGF-II levels because Igf2 is paternally expressed in the mouse embryo in most tissues. The expression of mesoderm markers and the subsequent formation of muscle structures were correlated with endogenous IGF-II level during teratoma formation and during in vitro differentiation. In addition, the absence of Igf2 in androgenetic Igf2 -/- ES cells led to a severe impairment of mesoderm development, demonstrating the dependence of the preferential mesoderm development of androgenetic ES cells upon Igf2 activity, among the numerous known imprinted genes. The addition of exogenous IGF-II to in vitro differentiation culture medium led to a specific increase in the expression of mesoderm markers. Thus, we propose a novel model in which the binding of IGF-II to its principal signaling receptor, IGF1R, at the surface of mesoderm precursor cells increases the formation of mesoderm cells. (C) 2000 Academic Press.