Interleukin-17 enhances bFGF-, HGF- and VEGF-induced growth of vascular endothelial cells
Interleukin-17 enhances bFGF-, HGF- and VEGF-induced growth of vascular endothelial cells
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DOI：
10.1016/j.imlet.2004.11.012
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发表时间：
2005-05-15
影响因子：
4.4
通讯作者：
Sasaki, H
中科院分区：
文献类型：
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作者：
Takahashi, H;Numasaki, M;Sasaki, H
干扰素-17（IL-17）是CD 4 T细胞衍生的促炎性和促血管生成细胞因子。在这项研究中，我们研究了这种细胞因子对由众所周知的直接血管生成因子bFGF、HGF、VEGF、CXCL 5/ENA-78或CXCL 8/IL-8诱导的血管内皮细胞生长的影响。虽然单独的广泛剂量的IL-17没有显示刺激人真皮微血管内皮细胞（HMVEC）生长的能力，但bFGF、HGF、VEGF、CXCL 5或CXCL 8在体外显著诱导HMVEC的生长。当bFGF和IL-17联合使用时，10或100 ng/ml IL-17增强10 ng/ml bFGF诱导的HMVECs生长。类似地，当HGF和IL-17组合在一起时，10或100 ng/ml IL-17增强10 ng/ml HGF诱导的HMVEC生长。当VEGF和IL-17一起使用时，10 ng/ml IL-17没有显著增强10 ng/ml VEGF诱导的生长，而100 ng/ml IL-17明显促进10 ng/ml VEGF介导的HMVEC增殖。相反，IL-17并没有增强CXCL 5和CXCL 8介导的生长。这些结果表明，IL-17本身不具有刺激血管内皮细胞生长的能力，而IL-17能够选择性地增强bFGF、HGF和VEGF对血管内皮细胞的促有丝分裂活性。我们的研究结果还表明，IL-17可能通过增强bFGF，HGF和VEGF诱导的血管内皮细胞生长，促进bFGF，HGF和VEGF介导的血管生成。(c)2004 Elsevier B. V.保留所有权利。
Interfeukin-17 (IL-17) is a CD4 T cell-derived proinflammatry and proangiogenic cytokine. In this study, we investigated the effects of this cytokine on vascular endothelial cell growth induced by a well-known direct angiogenic factor bFGF, HGF, VEGF, CXCL5/ENA-78 or CXCL8/IL-8. While a wide range of doses of IL-17 alone did not show the ability to stimulate the growth of human dermal microvascular endothelial cells (HMVECs), bFGF, HGF, VEGF, CXCL5 or CXCL8 significantly induced the growth of HMVECs in vitro. When bFGF and IL-17 were used in combination, 10 or 100 ng/ml IL-17 enhanced 10 ng/ml bFGF-induced growth of HMVECs. Similarly, when HGF and IL-17 were combined together, 10 or 100 ng/ml IL-17 potentiated 10 ng/ml HGF-induced growth of HMVECs. When VEGF and IL-17 were used together, 10 ng/ml IL-17 did not significantly enhance 10 ng/ml VEGF-induced growth, whereas 100 ng/ml IL-17 clearly promoted 10 ng/ml VEGF-mediated proliferation of HMVECs. On the contrary, IL-17 did not augment CXCL5- and CXCL8-mediated growth. These results indicate that IL-17 itself does not have the capability to stimulate the growth of vascular endothelial cells, whereas IL-17 is able to selectively enhance the mitogenic activity of bFGF, HGF, and VEGF for vascular endothelial cells. Our findings also suggest that IL-17 may promote bFGF-, HGF- and VEGF-meadiated angiogenesis through enhancing bFGF-, HGF- and VEGF-induced growth of vascular endothelial cells. (c) 2004 Elsevier B.V. All rights reserved.