IN-VITRO PRODUCTION OF CYTOKINES BY PERIPHERAL-BLOOD MONONUCLEAR-CELLS FROM HYDATID PATIENTS
IN-VITRO PRODUCTION OF CYTOKINES BY PERIPHERAL-BLOOD MONONUCLEAR-CELLS FROM HYDATID PATIENTS
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DOI：
10.1111/j.1365-2249.1995.tb05569.x
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发表时间：
1995-03-01
影响因子：
4.6
通讯作者：
SIRACUSANO, A
中科院分区：
文献类型：
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作者：
RIGANO, R;PROFUMO, E;SIRACUSANO, A
通过免疫测定评估了细胞因子在人体包虫病（细粒棘球绦虫感染）中的作用，该测定确定了30名包虫病患者和14名未感染对照者的外周血单个核细胞（PBMC）培养物中白细胞介素 - 4（IL - 4）、白细胞介素 - 10（IL - 10）和干扰素 - γ（IFN - γ）的产生情况。在来自包虫病患者的细胞培养物中，寄生虫抗原和非寄生虫抗原刺激显著增加了IL - 4的产生（P≤0.005）。患者和对照者的自发性和有丝分裂原驱动的IL - 4产生情况相似。两组中IL - 10和IFN - γ的产生在统计学上没有差异，尽管一些包虫病患者会大量产生这些细胞因子。值得注意的是，患者中抗原驱动的IFN - γ浓度始终高于未感染的对照者。数据分析显示IgE和IgG4反应与寄生虫驱动的细胞因子产生之间存在关联。高IgE和IgG4反应者产生高浓度的IL - 4和IL - 10。高IgE反应者的IFN - γ产生减少，但高IgG4反应者的IFN - γ水平略高于低反应者。细胞因子反应模式与疾病的临床阶段无关。本研究中许多包虫病患者的PBMC中发现的IL - 4显著增加以及高浓度的IL - 10与人体包虫病中Th2细胞的激活是一致的。细粒棘球绦虫感染患者中存在抗原驱动的IFN - γ产生意味着Th1或Th0细胞亚群的同时干预。
The role of cytokines in human hydatidosis (Echinococcus granulosus infection) was evaluated in immunoassays determining production of IL-4, IL-10 and interferon-gamma (IFN-gamma) in peripheral blood mononuclear cell (PBMC) cultures from 30 hydatid patients and 14 uninfected controls. In cell cultures from hydatid patients parasite and non-parasite antigen stimulation significantly increased IL-4 production (P less than or equal to 0.005). Spontaneous and mitogen-driven IL-4 production was similar in patients and controls. IL-10 and IFN-gamma production did not differ statistically in the two groups, even though some hydatid patients produced these cytokines in large amounts. Notably, antigen-driven IFN-gamma concentrations were invariably higher in patients than in uninfected controls. Data analysis showed a relationship between IgE and IgG4 responses and parasite-driven cytokine production. High IgE and IgG4 responders produced high IL-4 and IL-10 concentrations. High IgE responders showed decreased IFN-gamma production, but high IgG4 responders had IFN-gamma levels slightly higher than those of low responders. Cytokine response patterns did not relate to the clinical stage of disease. The significantly increased IL-4 and the high IL-10 concentrations found in PBMC from many hydatid patients in this study are consistent with Th2 cell activation in human hydatidosis. The presence of antigen-driven IFN-gamma production inpatients with E. granulosus infection implies concurrent intervention of the Th1 or Th0 cell subset.