Isolation and characterization of new wild-type isolates of bovine lentivirus.
Isolation and characterization of new wild-type isolates of bovine lentivirus.
复制标题
牛慢病毒新野生型分离株的分离和鉴定。
DOI：
10.1128/jvi.67.8.5051-5055.1993
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发表时间：
1993
影响因子：
5.4
通讯作者：
Whetstone,CA
中科院分区：
文献类型：
--
作者：
Suarez,DL;VanDerMaaten,MJ;Wood,C;Whetstone,CA
从佛罗里达州的一个血清反应呈阳性的牛群中获得了两种新的牛慢病毒分离株，也称为牛免疫缺陷样病毒（BIV）。这是自 1969 年分离原始 BIV 菌株 R29 以来首次报道 BIV 新分离株。这两种新 BIV 分离株源自与胎牛肺细胞培养物在体外共培养的血沉棕黄层细胞。在体外，新分离株在胎牛肺细胞中的复制和合胞体形成方面与原始 R29 分离株不同。通过免疫荧光测定、蛋白质印迹（免疫印迹）和聚合酶链反应，这两种新分离株均被确认为 BIV。聚合酶链式反应pol基因产物的序列分析显示与已发表的BIV R29-127（衍生自BIV R29的分子克隆）的核苷酸序列有92.6%和93.6%的同源性。将每种新的 BIV 分离株接种到两只小牛体内，并在接种后 5 至 10 天（接种后）恢复病毒，并在接种后 10 至 21 天之间进行 BIV 血清转化。注射后至少 4 个月内病毒可恢复且抗体可检测到。两只小牛的单核细胞计数出现短暂升高，类似于最初实验小牛接种中 BIV R29 的报告。没有观察到其他临床异常。
Two new isolates of bovine lentivirus, also known as bovine immunodeficiency-like virus (BIV), were obtained from a seropositive cattle herd in Florida. This is the first report of new isolates of BIV since the original BIV strain, R29, was isolated in 1969. The two new BIV isolates were derived from blood buffy coat cells cocultivated in vitro with fetal bovine lung cell cultures. The new isolates differed in vitro from the original R29 isolate in replication and syncytium formation in fetal bovine lung cells. Both new isolates were confirmed as BIV by immunofluorescence assay, Western blotting (immunoblotting), and polymerase chain reaction. Sequence analyses of the polymerase chain reaction pol gene product showed 92.6 and 93.6% homology to the published nucleotide sequence of BIV R29-127, a molecular clone derived from BIV R29. Each of the new BIV isolates was inoculated into two calves, and virus was recovered between 5 and 10 days postinoculation (p.i.), with BIV seroconversion between 10 and 21 days p.i. Virus was recoverable and antibody was detectable for at least 4 months p.i. Two calves developed a transiently elevated mononuclear cell count, similar to what was reported for BIV R29 in the original experimental calf inoculations. No other clinical abnormalities were observed.