Electrospun Nanofibers with Core-Shell Structure for Treatment of Bladder Regeneration
Electrospun Nanofibers with Core-Shell Structure for Treatment of Bladder Regeneration
复制标题
具有核壳结构的电纺纳米纤维用于治疗膀胱再生
DOI：
10.1089/ten.tea.2018.0255
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发表时间：
2019-03-18
影响因子：
4.1
通讯作者：
Zeng, Xiaoyong
中科院分区：
文献类型：
--
作者：
Feng, Chunxiang;Liu, Chang;Zeng, Xiaoyong
透明质酸（HA）是细胞外基质（ECM）的重要成分，静电纺丝支架具有与自体ECM相似的三维多孔网络结构特征。然而，没有表面活性剂的水溶性HA的支架制造是困难的，通过静电纺丝。本研究报道了通过同轴电纺聚（l-丙交酯）/聚（e-己内酯）（PLCL）和HA来制备HA涂层的仿生膀胱支架，以调节平滑肌细胞（SMC）的增殖和迁移，用于膀胱再生。通过扫描电镜、透射电镜、衰减全反射傅里叶变换红外光谱对纳米纤维进行了表征。测量接触角和溶胀度以确定纳米支架的亲水性。将SMC接种到PLCL/HA和PLCL支架上进行体外研究。对于体内研究，将36只大鼠分为PLCL/HA组、PLCL组、膀胱切开术对照组和假手术组，每组8只大鼠。进行了40-50%的超区细胞移植，并用或不用圆顶支架进行替换。对大鼠进行4周和10周的随访。随访后行尿动力学检查膀胱功能。切除缺损区域进行组织学检查。PLCL/HA纳米纤维具有高度均匀的核壳结构。PLCL纳米纤维上的HA涂层赋予纳米纤维垫增加的各向异性润湿性和溶胀性。PLCL/HA纳米纤维显著促进SMC增殖和迁移。在大鼠膀胱增大模型中的体内植入表明，由PLCL/HA支架制成的膀胱壁与宿主组织整合，并刺激表达收缩蛋白α-平滑肌肌动蛋白的膀胱平滑肌层的形成。此外，膀胱容量显著改善。这些结果表明，双层PLCL/HA圆顶网是一种有前途的支架，具有良好的细胞相容性，并能促进平滑肌组织增殖，用于膀胱再生。
Hyaluronic acid (HA) is an important component of the extracellular matrix (ECM), and electrospun scaffolds have the three-dimensional porous network structure characteristics similar to those of the autologous ECM. However, scaffold fabrication of water-dissolved HA without surfactant by electrospinning is difficult. This study reports the fabrication of HA-coated biomimetic nanofiber scaffolds through coaxial electrospinning of poly(l-lactide)/poly(e-caprolactone) (PLCL) and HA to modulate proliferation and migration of smooth muscle cells (SMCs) for bladder regeneration. The nanofibers were verified by scanning and transmission electron microscopy, attenuated total reflection Fourier transform infrared spectroscopy. Contact angle and swelling were measured to determine hydrophilicity of the nanofiber scaffolds. SMCs were seeded onto the PLCL/HA and PLCL scaffolds for the in vitro study. For the in vivo study, 36 rats were divided into the PLCL/HA, PLCL, cystotomy controls, and sham surgery groups, 8 rats per group. The 40-50% supratrigonal cystectomy was performed and replaced with or without domed scaffolds. The rats were followed for 4 and 10 weeks. After the follow-up, urodynamic test was performed to check the functions of bladder. The defect area was excised for histological examination. Highly uniform PLCL/HA nanofibers had the core-shell structures. The HA coating on the PLCL nanofibers endowed the nanofibrous mats with increased anisotropic wettability and swelling. The PLCL/HA nanofibers significantly promoted SMC proliferation and migration. In vivo implantation in a rat bladder augmentation model demonstrated that the bladder walls made of PLCL/HA nanofiber scaffolds integrated with host tissue and stimulated the formation of bladder smooth muscle layers that expressed contractile protein alpha-smooth muscle actin. In addition, bladder capacity was significantly improved. These findings show that the bilayer PLCL/HA nanofiber domed mesh is a promising scaffold with good cytocompatibility and can promote smooth muscle tissue proliferation for bladder regeneration.