ULTRASTRUCTURAL STUDY OF AFRICAN SWINE FEVER VIRUS REPLICATION IN CULTURES OF SWINE BONE-MARROW CELLS
ULTRASTRUCTURAL STUDY OF AFRICAN SWINE FEVER VIRUS REPLICATION IN CULTURES OF SWINE BONE-MARROW CELLS
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DOI：
10.1007/bf02175596
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发表时间：
1975-01-01
影响因子：
2.7
通讯作者：
TERRINHA, AM
中科院分区：
文献类型：
--
作者：
MOURANUNES, JF;VIGARIO, JD;TERRINHA, AM
用猪骨髓细胞培养物感染ASFV，研究其复制周期。在细胞质复制区有膜段分化。其中一些是多边形的，从而产生病毒颗粒。包括非多边形膜在内的病毒编码物质的过量生产似乎是该细胞系统中ASFV复制周期的一个重要特征。病毒被细胞膜包裹着释放。在细胞质中很少见到病毒的晶状排列。我们从来没有在细胞核中看到过病毒。感染细胞在感染后早期就表现出明显的细胞病变效应。
Cultures from pig bone marrow cells were infected with ASFV and the replication cycle was studied. In the cytoplasmic replication areas there are a differentiation of membrane segments. Some of them are polygonal, which give rise to virus particles. An over production of viral coded materials, including non-polygonal membranes seems to be an important feature of the replicative cycle of ASFV in this cell system. Viruses are released enveloped with a cellular membrane. Paracrystalline arrays of viruses are seldom seen in the cytoplasm. At no time did we see viruses in the nucleus. Infected cells showed marked cytopathic effect, beginning at early times post infection.