Lipid domain coarsening and fluidity in multicomponent lipid vesicles: A continuum based model and its experimental validation
Lipid domain coarsening and fluidity in multicomponent lipid vesicles: A continuum based model and its experimental validation
复制标题
多组分脂质囊泡中脂质结构域的粗化和流动性：基于连续介质的模型及其实验验证
DOI：
10.1016/j.bbamem.2022.183898
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发表时间：
2022-03-24
影响因子：
3.4
通讯作者：
Majd, S.
中科院分区：
文献类型：
--
作者：
Wang, Y.;Palzhanov, Y.;Majd, S.
Liposomes that achieve a heterogeneous and spatially organized surface through phase separation have been recognized to be a promising platform for delivery purposes. However, their design and optimization through experimentation can be expensive and time-consuming. To assist with the design and reduce the associated cost, we propose a computational platform for modeling membrane coarsening dynamics based on the principles of continuum mechanics and thermodynamics. This model couples phase separation to lateral flow and accounts for different membrane fluidity within the different phases, which is known to affect the coarsening dynamics on lipid membranes. The simulation results are in agreement with the experimental data in terms of liquid ordered domains area fraction, total domains perimeter over time, and total number of domains over time for two different membrane compositions (DOPC:DPPC with a 1:1 M ratio with 15% Chol and DOPC:DPPC with a 1:2 M ratio with 25% Chol) that yield opposite and nearly inverse phase behavior. This quantitative validation shows that the developed platform can be a valuable tool in complementing experimental practice.
