Determinants of rotavirus host range restriction - A heterologous bovine NSP1 gene does not affect replication kinetics in the pig
Determinants of rotavirus host range restriction - A heterologous bovine NSP1 gene does not affect replication kinetics in the pig
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DOI：
10.1006/viro.1998.9108
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发表时间：
1998-05-25
期刊：
影响因子：
3.7
通讯作者：
Howard, CR
中科院分区：
文献类型：
--
作者：
Bridger, JC;Dhaliwal, W;Howard, CR
轮状病毒宿主范围限制（宿主物种特异性）的遗传基础尚不清楚，但一些研究表明NSP1（第五）基因与之有关。我们研究了一种NSP1基因单重配体E11在体内的复制动力学，以评估异源NSP1基因对在猪体内复制能力的影响。该单重配体拥有来自猪源亲本轮状病毒SW20/21的10个基因，该病毒能在猪体内有效复制，以及来自牛轮状病毒UK的NSP1基因，牛轮状病毒UK在猪体内产生流产性感染。每组多达4头猪，分别口服接种10⁵到10⁶ TCID₅₀的单重配体、猪源亲本轮状病毒或牛源亲本轮状病毒，或进行假接种。单重配体在猪体内有效复制，其复制动力学与猪源亲本轮状病毒几乎相同。在接种后的9天观察期内，单重配体和猪源亲本轮状病毒在粪便中含病毒的天数、病毒排出的起始时间和持续时间以及粪便中的病毒滴度峰值都相似。通过聚丙烯酰胺凝胶电泳（PAGE）证实粪便中排出的病毒基因组成与接种物相同。因此，拥有来自在猪体内无法复制的牛轮状病毒的异源NSP1基因，既没有产生流产性感染，也没有影响体内的复制动力学。猪和牛轮状病毒之间宿主范围限制的遗传基础仍有待确定。（C）1998年学术出版社
The genetic basis of rotavirus host range restriction (host species specificity) is unknown but the NSP1 (fifth) gene has been implicated in some studies. We studied the replication kinetics in vivo of a NSP1 gene monoreassortant, E11, to assess the influence of a heterologous NSP1 gene on the ability to replicate in pigs, The monoreassortant possessed 10 genes from the porcine parent rotavirus SW20/21, which replicated productively in pigs, and the NSP1 gene from the bovine rotavirus UK which produced an abortive infection in pigs. Groups of up to four pigs;were inoculated orally with 10(5) to 10(6) TCID50 of the monoreassortant, the porcine parent rotavirus, or the bovine parent rotavirus or were sham inoculated. The monoreassortant replicated productively in pigs with replication kinetics almost identical to the porcine parent rotavirus. During a 9-day observation period after inoculation, the number of days with virus in the faeces, the onset and duration of virus excretion, and peak titres in faeces were similar for the monoreassortant and the parent porcine rotavirus. The genetic composition of the viruses excreted in the faeces was confirmed as that of the inocula by PAGE. Thus possession of a heterologous NSP1 gene from a bovine rotavirus which failed to replicate in pigs did not produce an abortive infection or affect the replication kinetics in vivo. The genetic basis of host range restriction between porcine and bovine rotaviruses remains to be established. (C) 1998 Academic Press.