Overexpression of the Tomato 13-Lipoxygenase Gene TomloxD Increases Generation of Endogenous Jasmonic Acid and Resistance to Cladosporium fulvum and High Temperature
Overexpression of the Tomato 13-Lipoxygenase Gene TomloxD Increases Generation of Endogenous Jasmonic Acid and Resistance to Cladosporium fulvum and High Temperature
复制标题
番茄 13-脂氧合酶基因 TomloxD 的过度表达可增加内源茉莉酸的产生以及对黄枝孢菌和高温的抵抗力
DOI：
10.1007/s11105-013-0581-4
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发表时间：
2013-10-01
影响因子：
2.1
通讯作者：
Chen, Guoping
中科院分区：
文献类型：
--
作者：
Hu, Tingzhang;Zeng, Hua;Chen, Guoping
番茄13-脂氧合酶基因TomloxD的表达受创伤、病原菌侵染、茉莉酸和系统素的刺激，但其在番茄生长发育中的作用不明显。目前仍不清楚。为了评价TomloxD的生理作用，我们在CaMV 35S启动子的控制下，利用正义构建法获得了TomloxD含量显著增加的转基因番茄植株。在转基因番茄中过表达TomloxD后，脂氧合酶活性和内源茉莉酸(JA)含量显著增加，表明TomloxD可以利用α-亚麻酸为底物合成(13S)-羟基过氧十八碳三烯酸(13S-HPOT)，并进一步代谢合成JA。实时荧光定量RT-PCR检测表明，转化植株中防御基因LeHSP90、LePR1、LePR6和LeZAT的表达水平均高于未转化植株。转TomloxD基因植株对病原菌和高温胁迫的抗性分析表明，转TomloxD基因植株比未转基因植株对叶霉病菌和高温胁迫的耐受性更强。这些数据清楚地表明，TomloxD参与了内源茉莉酸的合成和对生物和非生物胁迫的耐受。TomloxD基因在抗生物和/或非生物胁迫因子的工程作物中具有潜在的应用前景。
Expression of the tomato gene encoding 13-lipoxygenase,TomloxD, is stimulated by wounding, pathogen infection, jasmonate, and systemin, but its role during growth and development of tomato (Lycopersicon Spp.) remains unclear. To assess the physiological role of TomloxD, we produced transgenic tomato plants with greatly increased TomloxD content using sense constructs under the control of the CaMV 35S promoter. Overexpression of TomloxD in transgenic tomatoes led to a marked increase in the levels of lipoxygenase activity and content of endogenous jasmonic acid (JA), which suggested that TomloxD can use α-linolenic acid as a substrate to produce (13S)-hydroperoxyoctadecatrienoic acid (13-HPOT); the 13-HPOT produced appears to be metabolized further to synthesize JA. Real-time RT-PCR revealed that the expression levels of defense genes LeHSP90, LePR1, LePR6 and LeZAT in the transformants were higher than those in non-transformed plants. Assay for resistance to pathogenic fungus and high temperature stresses suggested that transgenic plants harboring TomloxD were more tolerant to Cladosporium fulvum and high temperature stress than non-transformed tomato plants. The data presented here indicate clearly that TomloxD is involved in endogenous JA synthesis and tolerance to biotic and abiotic stress. The tomloxD gene has potential applications in engineering cropping plants that are resistant to biotic and/or abiotic stress factors.