Characterization of plasma-stabilized liposomes composed of dioleoylphosphatidylethanolamine and oleic acid [published errtum appears in Biochem Biophys Res Commun 1989 Sep 29;163(3):1539].
Characterization of plasma-stabilized liposomes composed of dioleoylphosphatidylethanolamine and oleic acid [published errtum appears in Biochem Biophys Res Commun 1989 Sep 29;163(3):1539].
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由二油酰磷脂酰乙醇胺和油酸组成的血浆稳定脂质体的表征[已发表的错误出现在 Biochem Biophys Res Commun 1989 Sep 29;163(3):1539]。
DOI：
10.1016/0006-291x(89)91999-2
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发表时间：
1989
影响因子：
3.1
通讯作者：
Huang,L
中科院分区：
文献类型：
--
作者：
Liu,DX;Zhou,F;Huang,L
我们以前曾报道过，由二油酰磷脂酰乙醇胺和油酸组成的小单层脂质体（d ≤ 200 nm）可以通过与正常人血浆孵育来稳定（Liu和Huang，Biochemistry 1989，出版中）。稳定的脂质体即使在相对苛刻的条件下，如极端pH、高盐和胰蛋白酶处理下也非常稳定。荧光去偏振的二苯基己三烯表明，稳定的脂质体具有较高的微粘度的脂质核心，这并没有减少，即使大部分的蛋白质被胰蛋白酶去除后。这些数据表明，血浆蛋白插入到脂质双层可能是负责的稳定活动。静脉注射到小鼠体内后，与常规脂质体组合物相比，稳定的脂质体显示出对肝脏和脾脏相对较低的亲和力。
We have previously reported that small unilamellar liposomes (d≦200 nm) composed of dioleoylphosphatidylethanolamine and oleic acid can be stabilized by incubating with normal human plasma (Liu and Huang, Biochemistry 1989, in press). The stabilized liposomes were very stable even under relatively harsh conditions such as extreme pH, high salt and trypsin treatment. Fluorescence depolarization of diphenylhexatriene showed that the stabilized liposome had a high microviscosity in the lipid core, which did not decrease even after the majority of proteins were removed by trypsin. These data suggest that plasma proteins inserted into the lipid bilayer are probably responsible for the stabilization activity. After i.v. injection into mouse, stabilized liposomes showed a relatively low affinity to liver and spleen as compared to a conventional liposome composition.