Protocol for inducing inflammation and acute myelin degeneration in larval zebrafish.
Protocol for inducing inflammation and acute myelin degeneration in larval zebrafish.
复制标题
在幼斑马鱼中诱导炎症和急性髓鞘变性的方案。
DOI：
10.1016/j.xpro.2022.101134
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发表时间：
2022-03-18
期刊：
影响因子：
--
通讯作者：
Quintana FJ
中科院分区：
文献类型：
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作者：
Jaronen M;Wheeler MA;Quintana FJ
该方案详细描述了持续时间<10天的幼斑马鱼中炎症和急性髓鞘变性的诱导。我们描述了使用该模型来筛选候选化合物对炎症的影响，然后进行RNA分离和基于qPCR的基因表达定量。然后，我们概述了与化合物相关的机制的生物信息学分析的步骤。该方案可与药物和遗传靶向结合使用，以确定导致神经退行性变的途径。有关此配置文件的使用和执行的完整详细信息，请参阅。用于快速筛选候选化合物对斑马鱼炎症的影响的方案方案能够识别转录变化与候选药物相关的途径的生物信息学分析该方案详细描述了在持续时间<10天的幼斑马鱼中诱导炎症和急性髓鞘变性。我们描述了使用该模型来筛选候选化合物对炎症的影响，然后进行RNA分离和基于qPCR的基因表达定量。然后，我们概述了与化合物相关的机制的生物信息学分析的步骤。该方案可与药物和遗传靶向结合使用，以确定导致神经退行性变的途径。
This protocol details the induction of inflammation and acute myelin degeneration in larval zebrafish with a duration of <10 days. We describe the use of this model to screen the effects of candidate compounds on inflammation, followed by RNA isolation, and qPCR-based quantification of gene expression. We then outline the steps for bioinformatic analysis of the mechanisms associated with the compounds. This protocol can be used in combination with drugs and genetic targeting to identify pathways that contribute to neurodegeneration. For complete details on the use and execution of this profile, please refer to. Protocol for rapid screening of candidate compounds on inflammation in zebrafish Protocol enables the identification of transcriptional changes Bioinformatic analysis of the pathways associated with drug candidates This protocol details the induction of inflammation and acute myelin degeneration in larval zebrafish with a duration of <10 days. We describe the use of this model to screen the effects of candidate compounds on inflammation, followed by RNA isolation, and qPCR-based quantification of gene expression. We then outline the steps for bioinformatic analysis of the mechanisms associated with the compounds. This protocol can be used in combination with drugs and genetic targeting to identify pathways that contribute to neurodegeneration.