Membrane recycling in secretory cells: pathway to the Golgi complex.
Membrane recycling in secretory cells: pathway to the Golgi complex.
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分泌细胞中的膜回收：通往高尔基复合体的途径。
DOI：
10.1002/9780470720745.ch9
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Farquhar,MG
中科院分区：
文献类型：
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作者：
Farquhar,MG
用电子致密示踪剂（葡聚糖和阳离子化铁蛋白）研究了通过内吞作用从分泌细胞表面回收的膜所采取的途径。检查的细胞类型包括腮腺和泪腺的外分泌细胞、垂体前叶的内分泌细胞和淋巴结或骨髓瘤细胞系的免疫球蛋白分泌细胞。在所有情况下，当细胞在37 ℃下孵育时，示踪剂最初被内吞作用吸收，随后出现在堆叠的高尔基体池中、未成熟的分泌颗粒或空泡中以及溶酶体中。当骨髓瘤细胞进行放射性碘标记和放射自显影后的标记成分的命运后，表面膜成分的共价标记得到类似的结果。最初只标记细胞表面，放射自显影颗粒集中在质膜上。在37 ℃孵育后，一些标记的成分被内化（通过内吞作用），大多数内部放射自显影颗粒被发现位于高尔基池和相关的分泌泡上，这是唯一被显著标记的细胞器。结果表明，存在着相当大的膜交通从质膜堆叠高尔基池和形成分泌颗粒或空泡在所有这些类型的细胞。因此，膜是不断恢复从分泌细胞的细胞表面和漏斗通过高尔基复合体，此外，质膜到高尔基交通似乎代表了一个主要的路线，膜交通在分泌细胞。这种运输的很大一部分似乎与用于分泌产品包装的膜容器的回收有关。
The pathway taken by membrane that is recovered by endocytosis from the surface of secretory cells was investigated with electron-dense tracers (dextrans and cationized ferritin). The cell types examined included exocrine cells of the parotid and lacrimal glands, endocrine cells of the anterior pituitary gland, and immunoglobulinsecreting cells from lymph nodes or myeloma cell lines. In all cases, when the cells were incubated at 37 C the tracers were initially taken up by endocytosis and they later appeared in the stacked Golgi cisternae, in immature secretion granules or vacuoles and in lysosomes. Similar results were obtained after covalent labelling of surface membrane constituents when myeloma cells were radioiodinated and the fate of the labelled components was followed by autoradiography. Initially only the cell surface was labelled, and the autoradiographic grains were concentrated over the plasmalemma. After incubation at 37 C some of the labelled components were internalized (by endocytosis), and the majority of the internal autoradiographic grains were found over Golgi cisternae and over associated secretory vacuoles, which were the only organelles significantly labelled. The findings indicate the existence of considerable membrane traffic from the plasmalemma to the stacked Golgi cisternae and forming secretion granules or vacuoles in all these cell types. Membrane is thus continually recovered from the cell surface of secretory cells and funnelled through the Golgi complex; moreover, the plasmalemma-to-Golgi traffic appears to represent a major route of membrane traffic in secretory cells. A large portion of this traffic appears to be associated with the recycling of the membrane containers used in the packaging of secretory products.