Human myelogenous leukemia: enhanced clonal proliferation in the presence of phorbol diesters.
Human myelogenous leukemia: enhanced clonal proliferation in the presence of phorbol diesters.
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DOI：
10.1182/blood.v57.2.256.bloodjournal572256
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发表时间：
1981-02
期刊：
影响因子：
20.3
通讯作者：
H. Koeffler
中科院分区：
文献类型：
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作者：
H. Koeffler
佛波醇双酯，包括低浓度的12-0-十四烷酰基-佛波醇-13-乙酸酯（TPA），增强人髓性白血病细胞系（KG-1）的克隆增殖。TPA（1.0 × 10（-9）-5.0 × 10（-11）M）在亚最佳浓度的集落刺激因子（CSF）存在下使KG-1克隆生长增加4 - 10倍，在最大刺激浓度的CSF存在下使KG-1克隆生长增加2 - 4倍。TPA类似物增强KG-1克隆生长的能力证实了它们在小鼠中促进肿瘤的报道能力。在含有TPA的液体培养基中培养的KG-1细胞的条件培养基中仅检测到轻微的CSF活性。TPA不能增加CSF刺激的正常人模型的髓系克隆生长，以研究TPA对CSF刺激的生长的增强作用。
Phorbol diesters, including 12-0-tetradecanoyl-phorbol-13-acetate (TPA) at low concentrations, enhanced the clonal proliferation of a human myelogenous leukemia cell line (KG-1). TPA (1.0 X 10(-9) -5.0 X 10(-11) M) increased KG-1 clonal growth by four to tenfold in the presence of suboptimal concentrations of colony stimulating factor (CSF) and by two to fourfold in the presence of maximally stimulating concentrations of CSF. The ability of TPA analogs to enhance KG-1 clonal growth paralleled their reported ability to promote tumors in mice. Only slight CSF activity was detected in the conditioned medium from KG-1 cells cultured in liquid medium with TPA. TPA was unable to increase myeloid clonal growth of CSF-stimulated normal human model to study TPA enhancement of CSF-stimulated growth.