CLA1, a novel gene required for chloroplast development, is highly conserved in evolution
CLA1, a novel gene required for chloroplast development, is highly conserved in evolution
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DOI：
10.1046/j.1365-313x.1996.9050649.x
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发表时间：
1996-05-01
期刊：
影响因子：
7.2
通讯作者：
Leon, P
中科院分区：
文献类型：
--
作者：
Mandel, MA;Feldmann, KA;Leon, P
从拟南芥的T-DNA产生的文库中分离出一种命名为cla 1 -1的白化病突变体。在cla 1 -1植物中，叶绿体发育在早期阶段被阻止。CLA 1 -1植物在黄化和产生花色素苷的能力方面表现得与野生型相似，表明光信号转导途径似乎未受影响。遗传学和分子生物学分析表明，T-DNA插入对CLA 1基因的破坏是导致突变表型的原因。RNA表达模式表明，CLA 1是积极的光调控，它有不同的影响，一些核和叶绿体编码的光合基因的稳态RNA水平。CLA 1基因的功能尚不清楚，但它编码一种在光合细菌和植物进化中保守的蛋白质，是拟南芥叶绿体发育所必需的。
An albino mutant designated cla1-1 (for 'cloroplastos glterados', or 'altered chloroplasts') has been isolated from a T-DNA-generated library of Arabidopsis thaliana. In cla1-1 plants, chloroplast development is arrested at an early stage. cla1-1 plants behave like wild-type in their capacity to etiolate and produce anthocyanins indicating that the light signal transduction pathway seems to be unaffected. Genetic and molecular analyses show that the disruption of a single gene, CLA1, by the T-DNA insertion is responsible for the mutant phenotype. RNA expression patterns indicate that CLA1 is positively regulated by light and that it has different effects on the steady-state RNA levels of some nuclear- and chloroplast-encoded photosynthetic genes. Although the specific function of the CLA1 gene is still unknown, it encodes a novel protein conserved in evolution between photosynthetic bacteria and plants which is essential for chloroplast development in Arabidopsis.