Gastroprotective effect of orexin-A and heme oxygenase system
Gastroprotective effect of orexin-A and heme oxygenase system
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DOI：
10.1016/j.jss.2014.08.048
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发表时间：
2015-02-01
影响因子：
2.2
通讯作者：
Izgut-Uysal, V. Nimet
中科院分区：
文献类型：
--
作者：
Gemici, Burcu;Tan, Ruken;Izgut-Uysal, V. Nimet
背景：Orexin-A除了在摄食机制中发挥重要作用外，还对急性胃黏膜损伤的形成具有有效的胃保护作用。本研究的目的是研究给药食欲素a对缺血再灌注（I/R）胃损伤对胃组织血红素加氧酶(HO)-1和HO-2表达的影响。材料与方法：Wistar大鼠缺血30min，再灌注3h。在I/R期间以500 pmol/kg/min的剂量输注Orexin-A。体视显微镜下测量病变面积。采用分光光度法测定胃黏膜髓过氧化物酶活性和4-羟基辛烯醇-丙二醛含量，酶联免疫吸附法测定胃肿瘤坏死因子-a含量。Western blotting检测HO-1、HO-2的表达。结果：Orexin-A显著降低I/R诱导的胃损伤、髓过氧化物酶活性和I/R暴露的胃组织中4-羟基壬烯醇-丙二醛浓度。在胃I/R模型中，食欲素a的胃保护作用伴随着HO-2表达的升高和HO-1表达的降低。结论：Orexin-A对I/R胃黏膜具有保护作用，其作用机制与Orexin-A在增加HO-2表达的同时，减少胃组织中性粒细胞浸润和脂质过氧化有关。(C) 2015爱思唯尔公司版权所有。
Background: Orexin-A, besides playing an important role in the mechanism of food intake, exhibits a potent gastroprotective action against the formation of acute gastric mucosal injury. The aim of the present study was to determine the effect of administered orexin-A against ischemia-reperfusion (I/R)-induced gastric injury on the expression of heme oxygenase (HO)-1 and HO-2 in gastric tissue.Materials and methods: Wistar rats were subjected to 30 min of ischemia followed by 3 h reperfusion. Orexin-A was infused at a dose of 500 pmol/kg/min during the I/R period. The lesion area was measured by stereomicroscope. The myeloperoxidase activity and 4-hydroxinonenol-malondialdehyde content of gastric mucosa were evaluated spectrophotometrically, and the gastric tumor necrosis factor-a was measured by enzyme linked immune sorbent assay. The expression of HO-1 and HO-2 was determined by Western blotting analysis.Results: Orexin-A significantly decreased the I/R-induced gastric lesions, myeloperoxidase activity, and 4-hydroxinonenol-malondialdehyde concentration in gastric tissue exposed to I/R. The gastroprotective effect of orexin-A in gastric I/R model was accompanied by the increase in HO-2 expression and the decrease in HO-1 expression.Conclusions: Orexin-A exerts a protective action on gastric mucosa subjected to I/R, and this effect is associated with the reduction of neutrophil infiltration and lipid peroxidation in gastric tissue in addition to the increase in HO-2 expression due to the administration of orexin-A. (C) 2015 Elsevier Inc. All rights reserved.