ADRENERGIC-STIMULATION OF PLACENTAL PROGESTERONE PRODUCTION
ADRENERGIC-STIMULATION OF PLACENTAL PROGESTERONE PRODUCTION
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DOI：
10.1210/jcem-56-5-969
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发表时间：
1983-01-01
影响因子：
5.8
通讯作者：
ZELEZNIK, AJ
中科院分区：
文献类型：
--
作者：
CARITIS, SN;HIRSCH, RP;ZELEZNIK, AJ
将来自足月人胎盘的细胞维持在培养物中，并在 24 小时内监测黄体酮的产生。 β1-肾上腺素能受体激动剂多巴酚丁胺(10-5M)和β2-肾上腺素能受体激动剂特布他林(10-5M)使黄体酮产生增加36±。 19% 和 49 .+-.与对照组相比，分别为 8% (.+-.SE) (P < 0.001)。普萘洛尔 (10-5 M) 完全阻断两种药物的刺激作用。与对照相比，cAMP 类似物 8-溴-cAMP (0.5 mM) 也显着改变（增加）黄体酮产生（P < 0.001），但普萘洛尔并未在很大程度上阻断这种作用。与对照相比，α-肾上腺素能受体激动剂甲氧明(10-4-10-6 M)没有显着改变胎盘孕酮的产生，并且特布他林对孕酮产生的刺激作用没有受到用酚妥拉明阻断α-肾上腺素能受体的显着影响。胎盘黄体酮的产生可通过刺激β-肾上腺素受体而显着调节，但不能通过刺激α-肾上腺素受体。这种反应可能是由细胞内 cAMP 增加介导的。这些发现对于考虑胎盘的其他代谢功能和早产的治疗可能很重要。
Cells from term human placentas were maintained in culture, and progesterone production was monitored over 24 h. The .beta.1-adrenergic receptor agonist dobutamine (10-5 M) and the .beta.2-adrenergic receptor agonist terbutaline (10-5 M) increased progesterone production by 36 .+-. 19% and 49 .+-. 8% (.+-. SE), respectively, compared with that in controls (P < 0.001). Propranolol (10-5 M) completely blocked the stimulatory effects of both drugs. The cAMP analog 8-bromo-cAMP (0.5 mM) also significantly altered (increased) progesterone production compared with controls (P < 0.001), but this effect was not blocked to a significant degree by propranolol. The .alpha.-adrenergic receptor agonist methoxamine (10-4-10-6 M) did not significantly alter placental progesterone production compared with controls, and the stimulatory effect of terbutaline on progesterone production was not significantly affected by blockade of the .alpha.-adrenergic receptor with phentolamine. Placental progesterone production can be significantly modulated by stimulation of .beta.-adrenergic, but not by .alpha.-adrenergic, receptors. This response may be mediated by increased intracellular cAMP. These findings may be important in considering other metabolic functions of the placenta and the treatment of preterm labor.