The widespread human desmocollin Dsc2 and tissue-specific patterns of synthesis of various desmocollin subtypes.
The widespread human desmocollin Dsc2 and tissue-specific patterns of synthesis of various desmocollin subtypes.
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广泛存在的人类桥粒胶蛋白 Dsc2 和各种桥粒胶蛋白亚型的组织特异性合成模式。
DOI：
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发表时间：
1995
影响因子：
6.6
通讯作者：
W. Franke
中科院分区：
文献类型：
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作者：
U. Nuber;S. Schäfer;A. Schmidt;P. J. Koch;W. Franke
通过比较dna衍生的氨基酸序列和细胞类型特异性的合成模式，我们确定了desmocolin Dsc2是最广泛的，可能是普遍存在的desmocolin亚型。通过Northern blot分析和核糖核酸酶保护试验，我们发现在所有已知具有桥粒的不同人体组织、肿瘤和细胞系中，编码Dsc2的mRNA约为5.6 kb，不仅包括上皮细胞，还包括心肌细胞和淋巴结。相比之下，desmocolin亚型Dsc1和Dsc3仅在某些层状鳞状上皮中被检测到，Dsc1最明显地局限于表皮和淋巴结，以及某些癌症和由此衍生的细胞系。我们还确定，编码较大多肽a的Dsc2 mRNA剪接形式和编码较短的Dsc2b的Dsc2 mRNA剪接形式出现在所有不同的组织和细胞系中。我们还发现，某些细胞，如表皮角质形成细胞系HaCaT和外阴癌衍生细胞系A-431，可以持续合成一种以上的Dsc亚型。讨论了各种Dsg和Dsc亚型合成的细胞类型特异性模式与胚胎发生期间组织发育和恶性转化的关系，并提出了特异性Dsg和Dsc亚型的试剂在肿瘤诊断中的应用。
By comparison of the cDNA-derived amino acid sequences and the cell type-specific patterns of synthesis we have identified desmocollin Dsc2 as the most widespread, perhaps ubiquitous desmocollin subtype. Using Northern blot analyses and ribonuclease protection assays we have found an approximately 5.6 kb mRNA encoding Dsc2 in all the diverse human tissues, tumors and cell lines examined that are known to possess desmosomes, i.e. not only epithelial cells but also myocardiac cells and lymph nodes. By contrast, desmocollin subtypes Dsc1 and Dsc3 have been detected only in certain stratified squamous epithelia, with the most conspicuous restriction of Dsc1 to epidermis and--remarkably, but unexplained--lymph nodes, and in certain carcinomas and cell lines derived therefrom. We have also determined that both Dsc2 mRNA splice forms, the one encoding the larger polypeptide a and the one coding for the shorter Dsc2b, occur in all the diverse tissues and cell lines examined. We also show that certain cells such as the epidermal keratinocyte line HaCaT and the vulvar carcinoma-derived line A-431 continually synthesize more than one Dsc subtype. The cell type-specific patterns of synthesis of the various Dsg and Dsc subtypes are discussed in relation to tissue development during embryogenesis and to malignant transformations, and the utilization of reagents for the specific Dsg and Dsc subtypes in tumor diagnosis is proposed.