Proteomic based identification of manganese superoxide dismutase 2 (SOD2) as a metastasis marker for oral squamous cell carcinoma.
Proteomic based identification of manganese superoxide dismutase 2 (SOD2) as a metastasis marker for oral squamous cell carcinoma.
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发表时间：
2008-03
影响因子：
2.5
通讯作者：
Hui Ye;Anxun Wang;Bao-shiang Lee;Tianwei Yu;Shihu Sheng;Tingsheng Peng;Shen Hu;D. Crowe;Xiaofeng Zhou
中科院分区：
文献类型：
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作者：
Hui Ye;Anxun Wang;Bao-shiang Lee;Tianwei Yu;Shihu Sheng;Tingsheng Peng;Shen Hu;D. Crowe;Xiaofeng Zhou
转移是口腔鳞状细胞癌（OSCC）进展的关键事件。为了鉴定OSCC转移的蛋白质组学生物标志物，检测了具有不同转移潜能的3对OSCC细胞系（UM 1/UM 2、1386 Tu/1386 Ln、686 Tu/686 Ln）。在这3种细胞系中，如使用体外细胞侵袭测定所测量的，UM 1、1386 Ln和686 Ln分别表现出比它们的配对细胞系UM 2、1386 Tu和686 Tu更高程度的转移潜力。采用2D-PAGE/MS蛋白质组学方法对40个差异表达蛋白进行鉴定。通过免疫组织化学（IHC）方法使用独立的OSCC患者组织样本进一步研究选定的候选蛋白（超氧化物歧化酶2和热休克蛋白27）。与配对的原发性OSCC相比，在淋巴结转移性疾病中观察到锰超氧化物歧化酶2（SOD 2）的IHC染色的统计学显著增加。因此，我们的研究结果表明，SOD 2水平升高与口腔鳞癌的淋巴结转移有关，并可能为转移的诊断提供预测价值。
Metastasis is a critical event in oral squamous cell carcinoma (OSCC) progression. To identify proteomic biomarkers for OSCC metastasis, 3 paired OSCC cell lines (UM1/UM2, 1386Tu/1386Ln, 686Tu/686Ln) with different metastatic potential were examined. Among those 3 cell lines, UM1, 1386Ln and 686Ln exhibited a higher degree of metastatic potential than their paired cell lines UM2, 1386Tu and 686Tu, respectively, as measured using an in vitro cell invasion assay. A total of 40 differentially expressed proteins were identified using 2D-PAGE/MS proteomic approach. Selected protein candidates (superoxide dismutase 2 and heat shock protein 27) were further investigated by immuno-histochemistry (IHC) method using independent OSCC patient tissue samples. The statistically significantly increases in IHC staining for manganese superoxide dismutase 2 (SOD2) were observed in lymph node metastatic disease when compared with the paired primary OSCC. Our results thus indicated that elevated SOD2 levels is associated with lymph node metastasis in OSCC and may provide predictive values for diagnosis of metastasis.