Analysis of the Transcriptome of the Infective Stage of the Beet Cyst Nematode, H. schachtii.
Analysis of the Transcriptome of the Infective Stage of the Beet Cyst Nematode, H. schachtii.
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DOI：
10.1371/journal.pone.0147511
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Jones MG
中科院分区：
文献类型：
--
作者：
Fosu-Nyarko J;Nicol P;Naz F;Gill R;Jones MG
甜菜胞囊线虫是甜菜、芸苔及相关作物的主要根部害虫。对这一重要植物病原体的分子特征研究有限：鉴定其控制H.使用Roche GS FLX对schachtii进行测序。百分之九十七的读取（即，387，668）分别用CAP3和CLC Genomics PCR组装成37，345和47，263个重叠群。通过与其他线虫的基因和基因组序列以及公共数据库中注释的蛋白质进行比较，对转录本进行注释。注释的转录本更类似于胞囊线虫的序列，而不是苍白球胞囊线虫和根结线虫（根结线虫属）。对这些转录本的分析表明，2，918个转录本的子集对于自由生活和植物寄生线虫是常见的，这表明该子集参与一般线虫代谢和发育。还确定了一组148个重叠群和183个单例编码先前表征为植物寄生线虫的效应子的推定同源物：已知这些对于通过组织或从细胞中取食的迁移过程中的宿主植物的寄生是重要的，或者被认为参与逃避或调节宿主防御。此外，存在的序列从线虫病毒的建议。该转录组的测序和注释显著增加了H. schachtii，并确定基因启动承担所需的作用，在关键的寄生前和早期寄生后J2阶段。这些数据为鉴定潜在的基因靶点提供了新的信息，为将来保护易感作物免受H。沙赫蒂。
The beet cyst nematode, Heterodera schachtii, is a major root pest that significantly impacts the yield of sugar beet, brassicas and related species. There has been limited molecular characterisation of this important plant pathogen: to identify target genes for its control the transcriptome of the pre-parasitic J2 stage of H. schachtii was sequenced using Roche GS FLX. Ninety seven percent of reads (i.e., 387,668) with an average PHRED score > 22 were assembled with CAP3 and CLC Genomics Workbench into 37,345 and 47,263 contigs, respectively. The transcripts were annotated by comparing with gene and genomic sequences of other nematodes and annotated proteins on public databases. The annotated transcripts were much more similar to sequences of Heterodera glycines than to those of Globodera pallida and root knot nematodes (Meloidogyne spp.). Analysis of these transcripts showed that a subset of 2,918 transcripts was common to free-living and plant parasitic nematodes suggesting that this subset is involved in general nematode metabolism and development. A set of 148 contigs and 183 singletons encoding putative homologues of effectors previously characterised for plant parasitic nematodes were also identified: these are known to be important for parasitism of host plants during migration through tissues or feeding from cells or are thought to be involved in evasion or modulation of host defences. In addition, the presence of sequences from a nematode virus is suggested. The sequencing and annotation of this transcriptome significantly adds to the genetic data available for H. schachtii, and identifies genes primed to undertake required roles in the critical pre-parasitic and early post-parasitic J2 stages. These data provide new information for identifying potential gene targets for future protection of susceptible crops against H. schachtii.