HEPATIC TRANSPLANTATION SURVIVAL - CORRELATION WITH ADENINE-NUCLEOTIDE LEVEL IN DONOR LIVER
HEPATIC TRANSPLANTATION SURVIVAL - CORRELATION WITH ADENINE-NUCLEOTIDE LEVEL IN DONOR LIVER
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DOI：
10.1002/hep.1840080306
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发表时间：
1988-05-01
期刊：
影响因子：
13.5
通讯作者：
CLOUSE, ME
中科院分区：
文献类型：
--
作者：
LANIR, A;JENKINS, RL;CLOUSE, ME
验证了ATP含量和能量电荷较高的人供肝在肝移植后效果较好。在移植前立即从25个供体肝脏获得活检，并使用高效液相色谱分析腺嘌呤核苷酸。结果与器官组织学、移植功能及转归相关。ATP浓度显著升高(4.22 .+-。2.87 vs. 0.71。0.69 nmol / mg蛋白质，p < 0.01)， ADP(8.75。2.96 vs. 4.49。1.95 nmol / mg蛋白质，p < 0.01)，能量电荷(0.43。0.15 vs. 0.21。0.04, p < 0.02)，成功肝（n = 20）相对于失败肝（n = 5）。两组在AMP、黄嘌呤或次黄嘌呤方面没有发现显著差异，尽管衰竭肝脏的平均值更高。15例肝ATP浓度高于2 nmol / mg蛋白质、能量电荷高于0.3的受体恢复良好。其他5例成功患者ATP浓度较低(0.70 +-。0.39 nmol / mg蛋白质)和0.20 .+-。0.03)术后并发感染或长时间高胆红素血症。在5例肝功能衰竭患者中，所有患者的ATP含量和能量电荷均较低。其中3人接受了肝脏移植，2人在移植后不久死亡。研究表明高ATP含量与良好的移植后预后之间存在直接关系。
The hypothesis was tested that human donor livers with higher ATP content and energy charge achieve better results after hepatic transplantation. Biopsies were obtained from 25 donor livers immediately prior to implantation and analyzed for adenine nucleotides using high-performance liquid chromatography. The results were correlated with organ histology, transplant function and outcome. Significantly higher concentrations of ATP (4.22 .+-. 2.87 vs. 0.71 .+-. 0.69 nmoles per mg protein, p < 0.01), ADP (8.75 .+-. 2.96 vs. 4.49 .+-. 1.95 nmoles per mg protein, p < 0.01) and energy charge (0.43 .+-. 0.15 vs. 0.21 .+-. 0.04, p < 0.02) were found in successful (n = 20) relative to failed (n = 5) livers. No significant differences were found in AMP, xanthine or hypoxanthine for the two groups, although the average values were higher in failed livers. Fifteen recipients with liver ATP concentration above 2 nmoles per mg protein and energy charge above 0.3 recovered well. Five other successful patients with lower ATP concentration (0.70 .+-. 0.39 nmoles per mg protein) and energy charge (0.20 .+-. 0.03) had postoperative courses complicated by infection or prolonged hyperbilirubinemia. In five patients whose livers failed, all had low ATP content and energy charge. Of these, three received a replacement liver and two died shortly after the transplantation. The study demonstrates a direct correlation between high ATP content and good postransplant outcome.