Enhanced heat stable antifungal factor production by Lysobacter enzymogenes OH11 with cheap feedstocks: medium optimization and quantitative determination
Enhanced heat stable antifungal factor production by Lysobacter enzymogenes OH11 with cheap feedstocks: medium optimization and quantitative determination
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DOI：
10.1111/lam.12870
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发表时间：
2018-05-01
影响因子：
2.4
通讯作者：
Liu, F. -Q.
中科院分区：
文献类型：
--
作者：
Tang, B.;Zhao, Y. -C.;Liu, F. -Q.
热稳定抗真菌因子（HSAF）因其广泛的抗真菌活性和新颖的作用方式被认为是一种潜在的生物农药。然而，很少有研究报道在发酵过程中产生HSAF。因此，本研究以大豆粉、葡萄糖和CaCl2的浓度分别为800、789和072gl（-1）为适宜的营养成分，对培养基组成进行优化，以最大限度地提高产酶溶杆菌OH11的HSAF产量。同时，通过消除乳化问题，建立了HSAF产率的定量分析，优化培养基的HSAF产率最高为356341386 mg(-1)，是10% TSB培养基（2934 +/- 257mg(-1)）的12倍。此外，对该培养基的成本进行了评估，比10%的TSB低近31倍。研究表明，优化后的培养基不仅有效，而且经济。研究的意义和影响研究的意义和影响：热稳定抗真菌因子（HSAF）具有一种新的作用模式，具有强大而广泛的抗真菌活性。提高产量和降低原材料成本对未来HSAF的生产尤为重要，但这些研究未见相关报道。本研究旨在通过培养基优化，提高HSAF的廉价原料产量，为HSAF在生物防治中的应用奠定基础。
Heat stable antifungal factor (HSAF) is considered to be a potential biological pesticide due to its broad antifungal activity and novel mode of action. However, few studies have reported on HSAF production during fermentation. Thus, this work was executed to optimize the medium composition to maximize HSAF production by Lysobacter enzymogenes OH11, with soybean flour, glucose and CaCl2 identified as suitable nutrients with concentrations of 800, 789 and 072gl(-1) respectively. Simultaneously, the quantitative analysis of HSAF production was established by eliminating the emulsification problem, and the highest HSAF production was determined to be 356341386 mgl(-1) using the optimized medium, 12-fold higher than when using the 10% TSB medium (2934 +/- 257mgl(-1)). Furthermore, the cost of this medium was assessed and nearly 31-fold lower than that of 10% TSB. This study suggests that the optimized medium is not only effective but also economical for HSAF production.Significance and Impact of the StudySignificance and Impact of the Study: Heat stable antifungal factor (HSAF) exhibits a potent and broad antifungal activity with a novel mode of action. Increased production and reduced cost of raw materials are particularly important for the future production of HSAF, however, no report was involved in these studies. This study aimed to improve the production of HSAF with cheap raw materials through the medium optimization, which would lay the foundation for the application of HSAF in biological control.