CELL LINEAGE IN THE CEREBRAL-CORTEX OF THE MOUSE STUDIED INVIVO AND INVITRO WITH A RECOMBINANT RETROVIRUS
CELL LINEAGE IN THE CEREBRAL-CORTEX OF THE MOUSE STUDIED INVIVO AND INVITRO WITH A RECOMBINANT RETROVIRUS
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DOI：
10.1016/0896-6273(88)90163-8
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发表时间：
1988-10-01
期刊：
影响因子：
16.2
通讯作者：
SANES, JR
中科院分区：
文献类型：
--
作者：
LUSKIN, MB;PEARLMAN, AL;SANES, JR
为了分析小鼠大脑皮层的细胞谱系，我们用重组逆转录病毒感染祖细胞，然后用逆转录病毒基因产物鉴定感染细胞的后代。c&ice在体内或分离培养后感染E12-El4。在这两种情况下，几乎所有的克隆都含有神经元或神经胶质细胞，但不是两者都含有。因此，在皮层发育早期，神经元和胶质细胞谱系出现分化。为了分析克隆相关细胞在体内的分布，从连续切片重建克隆边界。围产期（E18-PNO），克隆相关细胞在向皮质板迁移时呈放射状排列。因此，克隆队列通过类似的径向路径。在迁移（PN7-PN23）后，神经元克隆通常保持放射状排列，而胶质克隆在取向上是可变的，这表明这两种细胞类型以不同的方式积累。神经元克隆有时跨越整个皮层。因此，单个祖细胞可以使神经元形成多个层。
To analyze cell lineage in the murine cerebral cortex, we infected progenitor cells with a recombinant retrovirus, then used the retroviral gene product to identify the descendants of infected cells. C&ices were infected on E12-El4 either in vivo or following dissociation and culture. In both cases, nearly all clones contained either neurons or glia, but not both. Thus, neuronal and glial lineages appear to diverge early in cortical development. To analyze the distribution of clonally related cells in vivo, clonal boundaries were reconstructed from serial sections. Perinatally (E18-PNO), clonally related cells were radially arrayed as they migrated to the cortical plate. Thus, clonal cohorts traverse a similar radial path. following migration (PN7-PN23), neuronal clones generally remained radially arrayed, while glial clones were variable in orientation, suggesting that these two cell types accumulate in different ways. Neuronal clones sometimes spanned the full thickness of the cortex. Thus, a single progenitor can contribute neurons to several laminae.