Emergence and recovery response of phosphate metabolites and intracellular pH in intact Mytilus edulis as examined in situ by in vivo 31P-NMR.
Emergence and recovery response of phosphate metabolites and intracellular pH in intact Mytilus edulis as examined in situ by in vivo 31P-NMR.
复制标题
通过体内 31P-NMR 原位检查，完整贻贝中磷酸盐代谢物和细胞内 pH 值的出现和恢复反应。
DOI：
10.1016/0167-4889(91)90176-x
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Macdonald,JM
中科院分区：
文献类型：
--
作者：
Fan,TW;Higashi,RM;Macdonald,JM
我们采用表面探针定位 31 P-NMR 光谱来原位检查短期浮出（缺氧）和重新沉没对完整贻贝中磷酸盐代谢物和细胞内 pH (pHi) 的影响。使用完整的生物体确保所有内在反应保持活跃，同时监测个体最大限度地减少随机行为和其他个体差异造成的不确定性。使用光刻、平衡匹配箔探针与 1 H-NMR 图像相结合，可以从后内收肌获取 31 P-NMR 光谱，并具有良好的信噪比。出苗后，所有贻贝均表现出 [Pi] 增加，[磷酸精氨酸] 和 pHi 下降，并且 [ATP] 随着时间的推移变化很小。 [磷酸精氨酸] 和 [Pi] 的互补行为表明了参与 [ATP] 维持的前体-产物关系，但 [磷酸精氨酸] 和 pHitime 过程之间的相似性不能那么容易解释。某些参数时间进程的不规则性可能反映了随机间隙活动。重新沉没反应表现出与浮出反应相反的情况，只是pH最终变成超碱性且有不规则的波动。这可能与“氧债”现象和氧化磷酸化增加有关。
We employed surface probe-localized31P-NMR spectroscopy to examine in situ the impact of short-term emergence (hypoxia) and resubmergence on phosphate metabolites and intracellular pH (pHi) in intact mussels. The use of intact organisms ensured that all intrinsic responses remained active while monitoring of individuals minimized uncertainties resulting from stochastic behavior and other individual differences. The use of a photoetched, balanced-match foil probe combined with1H-NMR images allowed31P-NMR spectra to be acquired from the posterior adductor muscle with good signal-to-noise. Upon emergence, all mussels exhibited an increase in [Pi], a decline in [phosphoarginine] and pHi, and very little changes in [ATP] with time. The complementary behavior of [phosphoarginine] and [Pi] indicated a precursor-product relationship involved in the maintenance of [ATP] but the similarity between [phosphoarginine] and pHitime-courses cannot be so readily explained. Irregularity in the time-courses of some parameters could have reflected stochastic gaping activity. Resubmergence responses exhibited a reversal of the emergence responses, except that the pHieventually became supraalkaline with irregular fluctuations. This might be related to the ‘oxygen debt’ phenomenon and increased oxidative phosphorylation.