Isolation of Pseudomonas aeruginosa from open ocean and comparison with freshwater, clinical, and animal isolates
Isolation of Pseudomonas aeruginosa from open ocean and comparison with freshwater, clinical, and animal isolates
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DOI：
10.1007/s00248-006-9059-3
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发表时间：
2007-02-01
影响因子：
3.6
通讯作者：
Kogure, Kazuhiro
中科院分区：
文献类型：
--
作者：
Khan, Nurul Huda;Shii, Yoshikazu;Kogure, Kazuhiro
铜绿假单胞菌是一种机会致病菌，导致人类、动物和植物的发病和死亡。这种细菌被认为广泛存在于陆地和淡水环境中，但不在开放的海洋环境中。我们的目的是澄清其存在于公海，其基因型和生理特征进行了比较，从临床，动物和淡水来源的分离株。水样采集自日本的淡水、海湾和近海环境。根据表型特征和BD Phoenix系统鉴定，62个分离物，其中26个来自公海，被鉴定为P. acruginosa。对所有菌株进行脉冲场凝胶电泳（PFGE），以及21个临床菌株和8个动物菌株。结果表明，外海菌株由几个基因型组成，这些基因型与其他菌株是分离的。虽然一些临床分离株形成簇，但其他菌株倾向于混合在一起。海洋分离物具有相似的PFGE和血清分型模式，观察到不同的Escherichia类型。一些是多重耐药的。通过实验室微宇宙研究，观察了铜绿假单胞菌对去离子水（DW）中NaCl浓度增加的反应。海洋菌株表现出更好的生存与增加，而河流和临床菌株受到抑制的增加。这些发现说明了开放海洋作为铜绿假单胞菌可能的储存库的潜在意义，并且可能存在这种环境所特有的克隆。据我们所知，这是第一个报告的存在和表征铜绿假单胞菌在开放的海洋。
Pseudomonas aeruginosa is an opportunistic pathogen responsible for morbidity and mortality in humans, animals, and plants. This bacterium has been regarded to be widely present in terrestrial and freshwater environments, but not in open ocean environments. Our purpose was to clarify its presence in open ocean, and their genotypic and physiological characteristics were compared with those of isolates from clinical, animal, and freshwater sources. Water samples were collected from freshwater, bays, and offshore environments in Japan. Sixty-two isolates, including 26 from the open ocean, were identified as P. acruginosa by phenotypic characteristics and the BD Phoenix System. Pulsed-field gel electrophoresis (PFGE) was performed on all strains, together with 21 clinical and 8 animal strains. The results showed that open ocean strains are composed of a few genotypes, which are separated from other strains. Although some clinical isolates made a cluster, other strains tended to mix together. Different antibiotypes were observed among marine isolates that had similar PFGE and serotyping patterns. Some were multidrug- resistant. Laboratory-based microcosm study were carried out to see the responses of P. aeruginosa toward increased NaCl concentrations in deionized water (DW). Marine strains showed better survival with the increase, whereas river and clinical strains were suppressed by the increase. These findings illustrate the potential significance of open ocean as a possible reservoir of P. aeruginosa, and there may be clones unique to this environment. To our knowledge this is the first report on the presence and characterization of P. aeruginosa in the open ocean.