Genomic characteristics of Staphylococcus aureus strains associated with high within-herd prevalence of intramammary infections in dairy cows
Genomic characteristics of Staphylococcus aureus strains associated with high within-herd prevalence of intramammary infections in dairy cows
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DOI：
10.3168/jds.2014-9074
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发表时间：
2015-10-01
影响因子：
3.5
通讯作者：
Luini, M.
中科院分区：
文献类型：
--
作者：
Cremonesi, P.;Pozzi, F.;Luini, M.
金黄色葡萄球菌是引起奶牛乳房炎的重要病原之一。根据以前的研究，Staph。具有不同致病性和传染性的金黄色葡萄球菌基因型可引起乳房内感染（IMI）并在同一牛群中共存。我们的研究旨在比较葡萄球菌。使用不同的分子方法，如核糖体间隔区（RS）-PCR，多位点序列分型（MLST），spa分型，核糖分型，脉冲场凝胶电泳（PFGE），和多重PCR，从不同的IMI流行率的牛群中分离金黄色葡萄球菌菌株。为此，31奶牛群与葡萄球菌。金黄色葡萄球菌IMI，并选择其中16个进行比较研究：8个高流行率（HP）牛群有葡萄球菌。金黄色葡萄球菌IMI患病率>28%，8个低患病率（LP）牛群的IMI患病率为
Staphylococcus aureus is one of the most important causes of mastitis in dairy cattle. Based on previous research, Staph. aureus genotypes with different pathogenic and contagious properties can cause intramammary infection (IMI) and coexist in the same herd. Our study aimed to compare Staph. aureus strains from herds that differed in IMI prevalence using different molecular approaches such as ribosomal spacer (RS)-PCR, multilocus sequence typing (MLST), spa typing, ribotyping, pulsed-field gel electrophoresis (PFGE), and multiplex PCR. For this purpose, 31 dairy herds with Staph. aureus IMI were selected, and 16 of these were chosen for a comparison study: the 8 high-prevalence (HP) herds had Staph. aureus IMI prevalence >28% and the 8 low-prevalence (LP) herds had an IMI prevalence