Production of transgenic deepwater indica rice plants expressing a synthetic Bacillus thuringiensis cryIA(b) gene with enhanced resistance to yellow stem borer.
Production of transgenic deepwater indica rice plants expressing a synthetic Bacillus thuringiensis cryIA(b) gene with enhanced resistance to yellow stem borer.
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DOI：
10.1016/s0168-9452(98)00053-3
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发表时间：
1998-06
期刊：
影响因子：
5.2
通讯作者：
Mohammad Firoz Alam;K. Datta;E. Abrigo;A. Vasquez;D. Senadhira;S. Datta
中科院分区：
文献类型：
--
作者：
Mohammad Firoz Alam;K. Datta;E. Abrigo;A. Vasquez;D. Senadhira;S. Datta
二化螟已被鉴定为深水水稻的主要害虫，造成严重的产量损失。苏云金芽孢杆菌的Bt基因(S)已被证明在害虫抗性规划中非常有效。利用表达Bt基因的转基因植物(S)是目前控制害虫危害的有效途径。我们利用生物遗传转化的方法，成功地将人工合成的creIA(B)基因导入深水水稻品种Vaidehi的胚性愈伤组织中。Southern和Western印迹分析证实Bt基因在再生植株中的存在和表达。在T1代中证实了转基因的遗传。昆虫生物测定表明，提高了对黄化螟的抗性。这是首次报道了转Bt基因的水稻。
Yellow stem borer has been identified as a major insect pest of deepwater rice, causing severe yield losses. Bt gene(s) from Bacillus thuringiensis have been proven very effective in pest resistance program. The use of transgenic plants expressing Bt gene(s) is now occupied effective approach to control insect infestation. We have successfully introduced a synthetic cryIA(b) gene into embryogenic calli of a deepwater indica rice variety, Vaidehi, by using the biolistic method of transformation. The presence and expression of the Bt gene in regenerated plants were confirmed by Southern and Western blot analyses. Inheritance of the transgene was confirmed in the T1generation. Insect bioassays showed an enhancement of resistance against the yellow stem borer. This is the first report of lowland rice, engineered with Bt gene.