Advances and applications of microcrystal electron diffraction (MicroED)
Advances and applications of microcrystal electron diffraction (MicroED)
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DOI：
10.1016/j.sbi.2023.102741
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发表时间：
2023-12-11
影响因子：
6.8
通讯作者：
Nannenga，Brent L.
中科院分区：
文献类型：
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作者：
Haymaker，Alison;Nannenga，Brent L.
微晶电子衍射（通常称为 MicroED）已成为高分辨率结构测定的有力工具。该方法利用低温透射电子显微镜从晶体中收集电子衍射数据，这些数据比其他传统衍射技术使用的数据小几个数量级。 MicroED 已用于多种样品，包括可溶性蛋白质、膜蛋白、有机小分子和材料。在这里，我们将回顾 MicroED 方法并重点介绍该方法的最新进展，并描述 MicroED 在结构生物学和化学晶体学领域的应用。
Microcrystal electron diffraction, commonly referred to as MicroED, has become a powerful tool for high-resolution structure determination. The method makes use of cryogenic transmission electron microscopes to collect electron diffraction data from crystals that are several orders of magnitude smaller than those used by other conventional diffraction techniques. MicroED has been used on a variety of samples including soluble proteins, membrane proteins, small organic molecules, and materials. Here we will review the MicroED method and highlight recent advancements to the methodology, as well as describe applications of MicroED within the fields of structural biology and chemical crystallography.