Focal hippocampal lesions induce seizures and long-lasting changes in mossy fiber enkephalin and CCK immunoreactivity.
Focal hippocampal lesions induce seizures and long-lasting changes in mossy fiber enkephalin and CCK immunoreactivity.
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DOI：
10.1016/0196-9781(88)90228-8
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发表时间：
1988-01-01
期刊：
影响因子：
3
通讯作者：
Lauterborn, J C
中科院分区：
文献类型：
--
作者：
Gall, C M;Pico, R M;Lauterborn, J C
齿状回门部的电解损伤已被证明可诱导行为性癫痫发作活动，并导致海马苔藓纤维系统中脑啡肽、胆囊收缩素和强啡肽免疫反应性的量的扰动。在本研究中，脑电图（EEG）记录，从海马对侧的门病变小鼠，证明存在的复发性海马癫痫发作活动，开始约一个小时postlesion，并持续几个小时后。行为癫痫发作被发现对应于癫痫样海马EEG的周期。对损伤后不同时间处死的动物对侧海马脑啡肽（ENK-I）和胆囊收缩素免疫反应性（CCK-I）的免疫细胞化学分析显示，ENK-I和CCK-I在损伤后6和12小时从苔藓纤维中耗尽，ENK-I在27小时恢复到超正常水平。ENK-I仍升高，而CCK-I在苔藓纤维系统中完全缺失。这些数据表明，提高的生理活动，在复发性边缘癫痫发作的形式，诱导持久的，但完全不同的改变，脑啡肽和CCK浓度在海马苔藓纤维系统。
Electrolytic lesions of the dentate gyrus hilus have been demonstrated to induce behavioral seizure activity and to result in perturbations in the amount of enkephalin, cholecystokinin, and dynorphin immunoreactivity in the hippocampal mossy fiber system. In the present study, electroencephalographic (EEG) recordings, made from hippocampus contralateral to a hilus lesion in mouse, demonstrate the presence of recurrent hippocampal seizure activity which begins approximately one hour postlesion and continues for several hours thereafter. Behavioral seizures were found to correspond to periods of epileptiform hippocampal EEG. Immunocytochemical analyses of enkephalin-(ENK-I) and cholecystokinin-immunoreactivity (CCK-I) in contralateral hippocampus of animals sacrificed at various postlesion intervals revealed that both ENK-I and CCK-I were depleted from the mossy fibers at 6 and 12 hr postlesion, and that ENK-I rebounded to supranormal levels by 27 hr. In two animals sacrificed 60 days following lesions which induced extreme behavioral seizure activity, ENK-I was still elevated while CCK-I was completely absent from the mossy fiber system. These data suggest that heightened physiological activity, in the form of recurrent limbic seizures, induces long-lasting but quite different alterations in enkephalin and CCK concentration in the hippocampal mossy fiber system.