Collagen scaffolds derived from a marine source and their biocompatibility
Collagen scaffolds derived from a marine source and their biocompatibility
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DOI：
10.1016/j.biomaterials.2006.01.015
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发表时间：
2006-05-01
期刊：
影响因子：
14
通讯作者：
Lee, YM
中科院分区：
文献类型：
--
作者：
Song, E;Kim, SY;Lee, YM
工业胶原蛋白的主要来源是小牛皮和骨头。然而，它们具有患牛海绵状脑病或传染性海绵状脑病的高风险。在这项研究中，从水母中提取了一种新型的酸溶性胶原蛋白，试图获得一种更安全的替代性胶原蛋白。通过冷冻干燥并与1-乙基-(3-3-二甲氨基丙基)碳二亚胺盐酸盐/N-羟基琥珀酰亚胺交联制备了由水母胶原蛋白组成的多孔支架，用于组织工程应用。水母胶原蛋白支架的酶降解动力学由 EDC/NHS 交联密度控制。 MTT 测定结果表明，水母胶原蛋白比其他天然来源的生物材料（包括牛胶原蛋白、明胶、透明质酸和葡聚糖）表现出更高的细胞活力。水母胶原蛋白支架还具有高度多孔且互连的孔结构，这对于高密度细胞接种、为三维基质中培养的细胞提供高效营养和氧气供应是有用的。为了确定与牛胶原蛋白或明胶诱导的炎症反应相比，水母胶原蛋白是否会引起我的特异性炎症反应，我们测量了促炎细胞因子和抗体分泌物的水平，并监测了体内植入后免疫细胞的群体变化。人们发现水母胶原蛋白诱导的免疫反应至少与牛胶原蛋白和明胶引起的免疫反应相当。 (c) 2006 Elsevier Ltd. 保留所有权利。
The primary sources of industrial collagens are calf skin and bone. However, these carry a high risk of bovine spongiform encephalopathy or transmissible spongiform encephalopathy. In this Study, a novel form of acid-soluble collagen was extracted from jellyfish in an effort to obtain an alternative and safer collagen. Porous scaffolds composed of jellyfish collagen were prepared by freeze-drying and cross-linking with 1-ethyl-(3-3-dimethylaminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide to be used in tissue engineering applications. Enzymatic degradation kinetics of jellyfish collagen scaffolds were controlled by EDC/NHS-cross-linking density. Results from an MTT assay indicated that jellyfish collagen exhibited higher cell viability than other naturally derived biomaterials, including bovine collagen, gelatin, hyaluronic acid, and glucan. Jellyfish collagen scaffolds also had a highly porous and interconnected pore structure, Which is useful for an high-density cell seeding, Lin efficient nutrient and an oxygen supply to the cells cultured in the three-dimensional matrices. To determine whether jellyfish collagen evokes my specific inflammatory response compared to that induced by bovine collagen or gelatin, we measured the levels of pro-inflammatory cytokines and antibody secretions and monitored the population changes of immune cells after in vivo implantation. Jellyfish collagen was found to induce an immune response at least comparable to those caused by bovine collagen and gelatin. (c) 2006 Elsevier Ltd. All rights reserved.