Discharge modulation of rat dorsal raphe neurons during sleep and waking:: effects of preoptic/basal forebrain warming
Discharge modulation of rat dorsal raphe neurons during sleep and waking:: effects of preoptic/basal forebrain warming
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DOI：
10.1016/s0006-8993(00)02561-0
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发表时间：
2000-09-01
期刊：
影响因子：
2.9
通讯作者：
McGinty, D
中科院分区：
文献类型：
--
作者：
Guzmán-Marín, R;Alam, MN;McGinty, D
在猫中，在整个睡眠-觉醒周期中，从中缝背核 (DRN) 记录的推定血清素能神经元 (PSN) 表现出所谓的快速眼动睡眠关闭 (REM-off) 放电模式。由于对行为大鼠 DRN 神经元的睡眠-觉醒放电模式知之甚少，本研究检查了该神经元群。本研究记录的 PSN 显示：（1）从清醒到 NREM 到 REM 睡眠的放电率逐渐降低； (2) 动作电位持续时间长，以及 (3) 全身施用选择性 5-HT1A 激动剂 (+/-)-8-羟基-2-(二正丙氨基) 四氢萘氢溴酸盐 (8-OH-DPAT) 后放电率降低。有证据支持这样的假设：NREM 睡眠受到位于视前区和邻近基底前脑 (POA/BF) 的温度调节机制的调节。我们之前报道过 POA/BF 变暖抑制下丘脑后部和基底前脑中促醒神经元的放电。由于 DRN 是脑干唤醒系统的组成部分并接收来自 POA/BF 的预测，因此我们研究了醒来时局部 POA/BF 升温 1.5-2.0 摄氏度对 DRN 神经元放电的影响。 POA/BF 变暖减少了 DRN 中 19 个 PSN 中的 14 个和其他 17 个与唤醒相关的神经元中的 12 个的放电。 DRN 神经元放电减少没有伴随脑电图频率或行为变化。这些结果表明，在不受约束和未麻醉的大鼠中，DRN 中记录的 PSN 表现出“唤醒活跃的 REM-off”放电模式，并进一步支持了这样的假设：POA/BF 温敏催眠系统通过协调抑制多个唤醒系统（包括由 DRN 调节的唤醒系统）来诱导睡眠。 (C) 2000 Elsevier Science B.V. 保留所有权利。
In cats, putative serotonergic neurons (PSNs) recorded from the dorsal raphe nucleus (DRN) across the sleep-wake cycle exhibit the so-called rapid eye movement sleep-off (REM-off) discharge pattern. Since, the sleep-wake discharge patterns of DRN neurons in behaving rats is poorly known, the present study examined this neuronal populations. The PSNs recorded in this study exhibited: (1) progressive decrease in discharge rate from waking to NREM to REM sleep; (2) long action potential duration, and (3) reduction of discharge rate after systemic administration of a selective 5-HT1A agonist, (+/-)-8-hydroxy-2-(di-n-propylamino) tetralin hydrobromide (8-OH-DPAT). Evidence supports the hypothesis that NREM sleep is modulated by thermoregulatory mechanisms localized in the preoptic area and adjacent basal forebrain (POA/BF). We previously reported that POA/BF warming suppresses the discharge of wake-promoting neurons in the posterior hypothalamus and the basal forebrain. Since the DRN is one component of the brainstem arousal system and receives projections from POA/BF, we examined the effects of local POA/BF warming by 1.5-2.0 degrees C during waking on the discharge of DRN neurons. POA/BF warming reduced the discharge in 14 of 19 PSNs and in 12 of 17 other wake-related neurons in the DRN. DRN neuronal discharge reduction occurred without accompanying EEG frequency or behavioral changes. These results suggest that PSNs recorded in DRN in unrestrained and unanesthetized rats exhibit a "wake-active REM-off" discharge pattern and further support the hypothesis that the POA/BF warm-sensitive hypnogenic system induces sleep by a coordinated inhibition of multiple arousal systems including that modulated by the DRN. (C) 2000 Elsevier Science B.V. All rights reserved.