Electrochemical Biosensor Using DNA Embedded Phosphorothioate Modified RNA for Mercury Ion Determination
Electrochemical Biosensor Using DNA Embedded Phosphorothioate Modified RNA for Mercury Ion Determination
复制标题
使用 DNA 嵌入硫代磷酸酯修饰 RNA 进行汞离子测定的电化学生物传感器
DOI：
10.1021/acssensors.7b00892
复制
发表时间：
2018-03-01
期刊：
影响因子：
8.9
通讯作者：
Liu, Gang
中科院分区：
文献类型：
--
作者：
Wang, Hui;Liu, Yang;Liu, Gang
汞（Hg）及其化合物广泛存在于自然环境中，对环境造成严重污染，对人类健康造成不可逆转的危害。快速准确的传感方法对于汞检测具有重要意义。在这里，利用汞（II）诱导的切割硫代磷酸（PS）修饰的RNA的无标记生物传感器。为了提高切割反应的产率，我们设计了一种特异性的单链DNA包埋有4个PS修饰的RNA（Hg-DPR），然后将Hg-DPR通过肽键与单壁碳纳米管场效应晶体管（SWNTs/FET）共价连接。在暴露于Hg（II）之后，Hg-DPR可以被有效地切割，这进一步导致单壁碳纳米管的导电性改变。利用相对电阻变化，Hg-DPR/SVVNTs/FET成功地检测到低至10 pM的Hg（II）水平，校准曲线在50 pM至100 nM和100 nM至10 μ M范围内呈线性。此外，Hg-DPR/单壁碳纳米管/场效应管表现出优异的灵敏度，便携性和低成本的Hg（II）检测。
Mercury (Hg) and its compounds, originating from a variety of natural and anthropogenic sources, are ubiquitous in the natural environment, and cause severe environmental contamination and pose irreversible harm to human health. A fast and accurate sensing approach is of significant importance for mercury detection. Here, a label-free biosensor using Hg(II)-induced cleavage of phosphorothioate (PS) modified RNA was exploited. We designed a specific single-stranded DNA embedded with four PS-modified RNA (Hg-DPR) to improve the cleavage reaction yield, and then Hg-DPR was covalently linked with single-walled carbon nanotube field effect transistor (SWNTs/FET) via a peptide bond. The Hg-DPR can be efficiently cleaved after exposure to Hg(II), which further causes the conductivity of the SWNTs to change. Using the relative resistance change, the Hg-DPR/SVVNTs/FET successfully detected Hg(II) levels as low as 10 pM, and the calibration curves were linear in the range of 50 pM to 100 nM and 100 nM to 10 mu M. Additionally, Hg-DPR/SWNTs/FET exhibited excellent sensitivity, portability, and low-cost for Hg(II) detection.