Dramatic enhancement of the specific expression of the heart-type fatty acid binding protein in rat brown adipose tissue by cold exposure
Dramatic enhancement of the specific expression of the heart-type fatty acid binding protein in rat brown adipose tissue by cold exposure
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DOI：
10.1016/s0014-5793(97)00619-4
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发表时间：
1997-06-30
期刊：
影响因子：
3.5
通讯作者：
Terada, H
中科院分区：
文献类型：
--
作者：
Daikoku, T;Shinohara, Y;Terada, H
为了了解棕色（BAT）和白色（WAT）脂肪组织能量代谢的差异，我们通过Northern blot分析检测了心脏型和脂肪型脂肪酸结合蛋白（H-FABP和A-FABP）的稳态转录水平。冷暴露后，大鼠BAT中H-FABP的转录量增加了约100倍，而WAT中H-FABP的转录量可以忽略不计，并且在冷暴露时仅略有增加。冷暴露对两种脂肪组织中H-FABP转录水平均无影响。此外，在褐脂肪细胞中，去甲肾上腺素（NE）处理可显著提高H-FABP转录水平，而A-FABP转录水平无变化。因此，冷暴露对BAT中H-FABP转录的刺激作用与解偶联蛋白（uncoupling protein， UCP）一样是由NE介导的，因此，H-FABP和UCP的表达可能受相同的机制控制。(C) 1997年欧洲生化学会联合会。
To understand the difference in energy metabolisms in brown (BAT) and white (WAT) adipose tissues, we examined the steady-state transcript levels of the heart-type and adipose-type fatty acid binding proteins (H-FABP and A-FABP, respectively) by Northern blot analysis, The transcript of H-FABP in rat BAT was increased about 100-fold by cold exposure, whereas that in WAT was negligible, and was increased only slightly by cold exposure, The transcript of A-FABP was observed in both BAT and WAT, the level being slightly greater in WAT, However, its transcript level was not affected by cold exposure in either adipose tissue, In addition, on treatment with norepinephrine (NE), transcript level of H-FABP was elevated markedly but that of A-FABP was not changed in rat brown adipocytes. Therefore, the stimulatory effect of cold exposure on the transcript of H-FABP in BAT was concluded to be mediated by NE, like that of the uncoupling protein (UCP), Thus, the expressions of H-FABP and UCP may be controlled by the same mechanism. (C) 1997 Federation of European Biochemical Societies.