Interactions between Exogenous FGF-2 and Sulfonic Groups: in Situ Characterization and Impact on the Morphology of Human Adipose-Derived Stem Cells
Interactions between Exogenous FGF-2 and Sulfonic Groups: in Situ Characterization and Impact on the Morphology of Human Adipose-Derived Stem Cells
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DOI：
10.1021/la400871c
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发表时间：
2013-06-25
期刊：
影响因子：
3.9
通讯作者：
Pashkuleva, Iva
中科院分区：
文献类型：
--
作者：
Amorim, Sara;Pires, Ricardo A.;Pashkuleva, Iva
FGF-2 is often used as a supplement to stem cells culture medium aiming at preserving their self-renewal capacity and plasticity through the passages. However, little is known on the influence of the underlying substrate in these interactions. In this study, we have used mixed self-assembled monolayers with different ratios of -SO3H and -OH tail groups to investigate the influence of substrate properties (e.g., charge) on the FGF-2 adsorption and activity. QCM-D data demonstrated that, in the presence of -OH groups, the quantity of the adsorbed FGF-2 is proportional to the percentage of surface -SO3H groups. The bioactivity of the adsorbed FGF-2 follows the same tendency as demonstrated by its interactions with anti-FGF-2. Surprisingly, the adlayer of FGF-2 formed on the surface containing only SO3H-tailed SAMs was similar to the surface with 25% of -SO3H groups, demonstrating that FGF-2 adsorption is not solely driven by electrostatic interactions. We related these results with changes in the morphology of adipose-derived stem cells (ASCs) cultured on the same surfaces.
