Identification and analysis of glycogen synthase kinase 3 beta1 interactome
Identification and analysis of glycogen synthase kinase 3 beta1 interactome
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糖原合成酶激酶 3 beta1 相互作用组的鉴定和分析
DOI：
10.1002/cbin.10095
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发表时间：
2013-08-01
影响因子：
3.9
通讯作者：
Liu, Langxia
中科院分区：
文献类型：
--
作者：
Gao, Xuejuan;Wang, Jian-Ying;Liu, Langxia
糖原合成酶激酶-3 β（GSK 3）最初被鉴定为葡萄糖代谢中的关键蛋白。GSK 3可能参与细胞的生长、运动和凋亡。系统鉴定GSK 3相关蛋白对于全面了解其功能至关重要。利用GST pull-down实验和LCMS/MS分析结合生物信息学工具，我们在肝癌HepG 2细胞中鉴定了114个与GSK 31相互作用的蛋白。大多数已鉴定的蛋白质参与代谢过程，而其他蛋白质对细胞增殖或迁移很重要，并且与癌症的发展和转移有关。在HepG 2细胞中，通过免疫共沉淀法证实了几种具有代表性的蛋白如hnRNPK、PCNA、Ezrin和STAT 1与GSK-31相互作用。对这些相互作用的进一步研究可能会发现GSK-31在肿瘤生长和转移中的确切作用和潜在机制。
Glycogen synthase kinase-3 beta (GSK3) was initially identified as a key protein in glucose metabolism. GSK3 might be involved in cell growth, motility and apoptosis. Systematic identification of GSK3-associated proteins is crucial for the exhaustive understanding of its functions. Using GST pull-down experiment and LCMS/MS analysis coupled to bioinformatics tools, we have identified 114 proteins that interacted with GSK31 in hepatocellular carcinoma HepG2 cells. Most of the identified proteins are implicated in metabolic process, whereas other proteins are important for cell proliferation or migration, and have been associated with cancer development and metastasis. Several representative proteins, such as hnRNPK, PCNA, Ezrin and STAT1, have been confirmed to interact with GSK-31 by co-immunoprecipitation in HepG2 cells. Further studies of these interactions may discover the precise roles and the underlying mechanisms of GSK-31 in tumour growth and metastasis.