NUCLEAR-LOCALIZATION OF FOAMY VIRUS GAG PRECURSOR PROTEIN
NUCLEAR-LOCALIZATION OF FOAMY VIRUS GAG PRECURSOR PROTEIN
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DOI：
10.1128/jvi.68.8.4946-4954.1994
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发表时间：
1994-08-01
影响因子：
5.4
通讯作者：
RETHWILM, A
中科院分区：
文献类型：
--
作者：
SCHLIEPHAKE, AW;RETHWILM, A
当感染的细胞与来自感染宿主的血清反应时，所有泡沫病毒都会引起强烈的核染色。这种核荧光将泡沫病毒与所有其他逆转录病毒区分开来。本文报道的实验表明，泡沫病毒Gag前体蛋白瞬时位于感染细胞的细胞核中，这可能是典型的泡沫病毒核荧光的原因。通过使用牛痘病毒表达系统，泡沫病毒Gag蛋白的核衣壳结构域中的保守的基本序列基序被鉴定为负责gag前体分子的核转运。这个基序也被发现能够指导异源蛋白质，人类免疫缺陷病毒的Gag蛋白，进入细胞核。
All foamy viruses give rise to a strong nuclear staining when infected cells are reacted with sera from infected hosts. This nuclear fluorescence distinguishes foamy viruses from all other retroviruses. The experiments reported here indicate that the foamy virus Gag precursor protein is transiently located in the nuclei of infected cells and this is the likely reason for the typical foamy virus nuclear fluorescence. By using the vaccinia virus expression system, a conserved basic sequence motif in the nucleocapsid domain of foamy virus Gag proteins was identified to be responsible for the nuclear transport of the gag precursor molecule. This motif was also found to be able to direct a heterologous protein, the Gag protein of human immunodeficiency virus, into the nucleus.