Metabolomics analysis of Pulsatilla decoction on treatment of wetness-heat-induced diarrhea in rats based on UPLC-Q/TOF-MS/MS
Metabolomics analysis of Pulsatilla decoction on treatment of wetness-heat-induced diarrhea in rats based on UPLC-Q/TOF-MS/MS
复制标题
基于UPLC-Q/TOF-MS/MS的白头翁汤治疗大鼠湿热腹泻的代谢组学分析
DOI：
10.1002/bmc.4629
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发表时间：
2019-08-13
影响因子：
1.8
通讯作者：
Wei, Yan-ming
中科院分区：
文献类型：
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作者：
Hua, Yong-li;Ma, Qi;Wei, Yan-ming
白头翁汤是治疗湿热腹泻的经典中药方剂。为了研究PD对WHD的治疗作用并阐明其可能的机制，我们采用了基于超高效液相色谱-四极杆飞行时间/质谱（UPLC-Q/TOF-MS/MS）的代谢组学策略，分析了32只大鼠的血清样本，以确定PD治疗WHD的差异代谢物和相关途径。在正交偏最小二乘判别分析（OPLS-DA）模型中，>= 1.0,FC >= 1.2或
Pulsatilla decoction (PD) is a classical prescription in traditional Chinese medicine that has therapeutic effects on wetness-heat-induced diarrhea (WHD). To investigate the therapeutic effects of PD in the treatment of WHD and elucidate the potential mechanism, we used a metabolomics strategy on the base of ultraperformance liquid chromatography coupled with quadrupole time-of-flight/mass spectrometry (UPLC-Q/TOF-MS/MS) and analyzed the serum samples of 32 rats to identify differential metabolites and pathways associated with the PD treatment of WHD. With variable importance for projection >1.0 in the Orthogonal partial least-squares discriminant analysis (OPLS-DA ) models and FC >= 1.2 or