AN ACTIVATED LCK TRANSGENE PROMOTES THYMOCYTE DEVELOPMENT IN RAG-1 MUTANT MICE
AN ACTIVATED LCK TRANSGENE PROMOTES THYMOCYTE DEVELOPMENT IN RAG-1 MUTANT MICE
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DOI：
10.1016/1074-7613(94)90077-9
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发表时间：
1994-07-01
期刊：
影响因子：
32.4
通讯作者：
TONEGAWA, S
中科院分区：
文献类型：
--
作者：
MOMBAERTS, P;ANDERSON, SJ;TONEGAWA, S
T细胞受体P（TCR β）链的表达对于主要α β胸腺细胞谱系中从CD 4（-）CD 8（-）阶段的转变是必需的。蛋白酪氨酸激酶p56（lck）参与了早期胸腺细胞分化和TCR β基因座等位基因排斥的调节。使用过表达激活的lck转基因的小鼠和lck基因被破坏的小鼠，我们证明了p5（lck）参与调节CD 4（+）CD 8（+）胸腺细胞库扩增至野生型水平的途径。此外，p56（lck）可能参与了CD 4（+）CD 8（+）胸腺细胞上推定的pre-TCR的下调。
Expression of the T cell receptor P (TCR beta) chain is necessary for the transition from the CD4(-)CD8(-) stage in the major alpha beta thymocyte lineage. The protein tyrosine kinase p56(lck) has been implicated in the regulation of early thymocyte differentiation and of allelic exclusion at the TCR beta locus. Using mice overexpressing an activated lck transgene and mice with a disruption of the lck gene, we demonstrate that p5(lck) participates in a pathway that regulates the expansion of the pool of CD4(+)CD8(+) thymocytes to wild-type levels. In addition, p56(lck) may be involved in the down-regulation of the putative pre-TCR on CD4(+)CD8(+) thymocytes.