Association between Coxiella burnetii shedding in milk and subclinical mastitis in dairy cattle
Association between Coxiella burnetii shedding in milk and subclinical mastitis in dairy cattle
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DOI：
10.1051/vetres:2007060
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发表时间：
2008-05-01
影响因子：
4.4
通讯作者：
Schukken, Ynte
中科院分区：
文献类型：
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作者：
Barlow, John;Rauch, Brad;Schukken, Ynte
本研究的目的是探讨贝氏柯克斯体在牛奶中脱落与奶牛慢性隐性乳房炎之间的潜在联系。在两项独立的研究中，我们确定了基于PCR的C。牛奶中的贝氏体和泌乳奶牛的慢性隐性乳房炎。这些研究是在一个商业奶牛群中进行的，其中通过需氧细菌学对亚临床乳腺炎进行了持续的密集监测，但没有对C.贝氏体感染在一项病例对照研究中，C区。通过实时定量PCR（RT-qPCR）确定的贝氏体状态与通过乳汁体细胞计数测量的慢性亚临床乳腺炎密切相关。在随后的横断面研究中，325头泌乳奶牛中有147头（45%）为C阳性。通过RT-qPCR检测复合乳样品中的贝氏体。在广义线性模型中，考虑到包括需氧性乳房内感染状态在内的协变量的影响，C。贝氏PCR状态是线性体细胞计数评分的显著预测因子。本研究结果与以往的少数报道一致，为乳腺癌可能存在乳腺特异性表现提供了证据。奶牛的贝氏体感染。
The objective of this research was to explore the potential association between Coxiella burnetii shedding in milk and chronic subclinical mastitis in dairy cattle. In two separate studies, we identified an association between PCR-based detection of C. burnetii in milk and chronic subclinical mastitis in lactating dairy cows. These studies were conducted in a commercial dairy herd where there was ongoing intensive monitoring of subclinical mastitis by aerobic bacteriology, but no prior knowledge or management of C. burnetii infections. In a case-control study, quarter level C. burnetii status determined by real-time quantitative PCR (RT-qPCR) was strongly associated with chronic subclinical mastitis as measured by milk somatic cell counts. In a subsequent cross sectional study, 147 (45%) of 325 lactating cows were positive for C. burnetii by RT-qPCR of composite milk samples. In a generalized linear model, accounting for the effect of covariates including aerobic intramammary infection status, C. burnetii PCR status was a significant predictor of linear somatic cell count score. In agreement with a small number of previous reports, this research provides evidence that there may be mammary gland specific manifestations of C. burnetii infections in dairy cattle.