A U87-EGFRvIII cell-specific aptamer mediates small interfering RNA delivery.
A U87-EGFRvIII cell-specific aptamer mediates small interfering RNA delivery.
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U87-EGFRvIII 细胞特异性适体介导小干扰 RNA 递送。
DOI：
10.3892/br.2014.276
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发表时间：
2014-07
期刊：
影响因子：
--
通讯作者：
Shi, Yusheng
中科院分区：
文献类型：
--
作者：
Tan, Yan;Wu, Xidong;Li, Shuji;Shi, Yusheng
U87-EGFRvIII is a U87 glioma cell line that overexpresses epidermal growth factor receptor variant III (EGFRvIII). In the present study, we investigated whether a DNA aptamer selected against U87-EGFRvIII using cell-based systematic evolution of ligands by exponential enrichment (cell-SELEX) could deliver c-Met small interfering RNA (siRNA) into U87-EGFRvIII cells and silence the targeted gene expression. The selected biotinylated aptamer (BA) was coupled to biotinylated c-Met siRNA by streptavidin to deliver siRNA into U87-EGFRvIII cells. c-Met siRNA, transfected with lipofectamine 2000, served as a positive control, while control siRNA, transferred with BA, served as a negative control. Western blotting was performed to detect changes in the c-Met protein expression, and MTT and Annexin V-fluorescein isothiocyanate/propidium iodine assays were used to determine changes in the proliferation and apoptosis of U87-EGFRvIII cells, respectively. Similar to the liposome-mediated group, U87-EGFRvIII cells that were transfected with BA-c-Met siRNA experienced a significant decrease in the c-Met protein expression (P<0.05). There were also significant increases in the apoptotic rate (P<0.05) and inhibition rate of cell growth (P<0.01) compared with the negative control group, indicating that BA could deliver c-Met siRNA into U87-EGFRvIII and result in target gene silencing. In conclusion, the results demonstrated that this DNA aptamer, obtained through cell-SELEX, can be used as an efficient and targeted carrier for siRNA delivery, providing a novel approach and strategy for the targeted combination therapy of glioblastoma.
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影响因子：
3.2
作者：
Huang, Yu-Fen;Shangguan, Dihua;Liu, Haipeng;Phillips, Joseph A.;Zhang, Xiaoling;Chen, Yan;Tan, Weihong
通讯作者：
Tan, Weihong
影响因子：
46.9
作者：
Dassie, Justin P.;Liu, Xiu-ying;Thomas, Gregory S.;Whitaker, Ryan M.;Thiel, Kristina W.;Stockdale, Katie R.;Meyerholz, David K.;McCaffrey, Anton P.;McNamara, James O., II;Giangrande, Paloma H.
通讯作者：
Giangrande, Paloma H.
适体介导的siRNA传递。
影响因子：
14.9
作者：
Chu, Ted C.;Twu, Karen Y.;Ellington, Andrew D.;Levy, Matthew
通讯作者：
Levy, Matthew
影响因子：
14.9
作者：
Zhou J;Swiderski P;Li H;Zhang J;Neff CP;Akkina R;Rossi JJ
通讯作者：
Rossi JJ
影响因子：
14.9
作者：
Thiel KW;Hernandez LI;Dassie JP;Thiel WH;Liu X;Stockdale KR;Rothman AM;Hernandez FJ;McNamara JO 2nd;Giangrande PH
通讯作者：
Giangrande PH